In the search for alternative therapy for infections and other ailments, metallic nanoparticles, mainly silver nanoparticles (AgNPs) synthesized through bioengineered sources are extensively explored. Fungal bioactive compounds and their nanoparticles were reported with the potential biomedical application. A medicinal mushroom Ganoderma lucidum was reported as a repository of rich medicinal properties. In the current study, silver nanoparticles were synthesized using the extracts of G. lucidum and its antimicrobial activity was tested against drug-resistant Escherichia coli isolated from the catheter used for urinary tract infection (CAUTI). The GC–MS study of G. lucidum extracts showed the presence of ethyl acetoacetate ethylene acetal with the highest area percentage of 72.2% and retention time (RT 5873). Pyridine-3-ol is the second primary compound with a peak height of 6.44% and a retention time of 2.143. The third compound is l,4-Dioxane-2,3-diol, with an area of 8.09% and RT 5450. Butylated Hydroxy Toluene [BHT] is the fourth major compound with an area of 3.32%, and 9-Cedranone constitutes the fifth position in occupying the area percentage [1.88] and height 1.56%. Pyrrole is the sixth primary compound registering an area size of 0.96% and height 2.06%. The AgNPs synthesized using G. lucidum extract were in size range 23 and 58 nm as per SEM analysis and within the range wavelength 0.556–0.796 nm as per UV–Vis spectral study. FTIR Spectroscopy and X-ray diffraction analysis (XRD) were made to characterize the formed nanoparticles. The AgNPs synthesized effectively inhibited the growth of E. coli isolated from catheter-associated urinary tract infection and showed resistance to many drugs. The antioxidant potential of the synthesized nanoparticles assessed using DPPH radical scavenging activity, EC50 (µg/ml), and ARP data showed that the prepared nanoparticles were more potent in free radical scavenging activity than the standard quercetin. The cytotoxicity effect of Ag-NPs on breast cancer cell line- MDA-MB-231 confirmed its anticancer potential. The half-maximal inhibitory concentration (IC₅₀) of Ag-NPs to inhibit 50% of the tumor was 9.2 g/mL. The synthesized GL-AgNPs was exhibited a multifocal biomedical potential.

在寻找感染和其他疾病的替代疗法时，广泛探索了金属纳米颗粒，主要是通过生物工程来源合成的银纳米颗粒 (AgNPs)。真菌生物活性化合物及其纳米颗粒被报道具有潜在的生物医学应用。据报道，一种药用蘑菇灵芝作为丰富的药用特性的储存库。在目前的研究中，使用灵芝的提取物合成了银纳米颗粒，并针对从用于尿路感染 (CAUTI) 的导管中分离出的耐药大肠杆菌进行了抗菌活性测试。灵芝的 GC-MS 研究提取物显示存在乙酰乙酸乙酯乙缩醛，其面积百分比最高，为 72.2%，保留时间 (RT 5873)。吡啶-3-醇是第二种主要化合物，峰高为 6.44%，保留时间为 2.143。第三个化合物是 l,4-Dioxane-2,3-diol，面积为 8.09%，RT 为 5450。丁基羟基甲苯 [BHT] 是第四个主要化合物，面积为 3.32%，9-Cedranone 构成第五位的占据面积百分比[1.88]和身高1.56%。吡咯是面积为 0.96% 和高度为 2.06% 的第六种主要化合物。使用灵芝合成的 AgNPs根据 SEM 分析，提取物的尺寸范围为 23 和 58 nm，根据 UV-Vis 光谱研究，提取物的波长范围为 0.556-0.796 nm。进行 FTIR 光谱和 X 射线衍射分析 (XRD) 以表征形成的纳米颗粒。合成的 AgNPs 有效抑制了从导管相关尿路感染中分离出来的大肠杆菌的生长，并对多种药物表现出抗性。使用 DPPH 自由基清除活性、EC50 (µg/ml) 和 ARP 数据评估的合成纳米颗粒的抗氧化潜力表明，制备的纳米颗粒在自由基清除活性方面比标准槲皮素更有效。Ag-NPs 对乳腺癌细胞系 MDA-MB-231 的细胞毒性作用证实了其抗癌潜力。半数最大抑制浓度（IC₅₀ ) 抑制 50% 肿瘤的 Ag-NPs 为 9.2 g/mL。合成的 GL-AgNPs 表现出多焦点生物医学潜力。