In the search for alternative therapy for infections and other ailments, metallic nanoparticles, mainly silver nanoparticles (AgNPs) synthesized through bioengineered sources are extensively explored. Fungal bioactive compounds and their nanoparticles were reported with the potential biomedical application. A medicinal mushroom Ganoderma lucidum was reported as a repository of rich medicinal properties. In the current study, silver nanoparticles were synthesized using the extracts of G. lucidum and its antimicrobial activity was tested against drug-resistant Escherichia coli isolated from the catheter used for urinary tract infection (CAUTI). The GC–MS study of G. lucidum extracts showed the presence of ethyl acetoacetate ethylene acetal with the highest area percentage of 72.2% and retention time (RT 5873). Pyridine-3-ol is the second primary compound with a peak height of 6.44% and a retention time of 2.143. The third compound is l,4-Dioxane-2,3-diol, with an area of 8.09% and RT 5450. Butylated Hydroxy Toluene [BHT] is the fourth major compound with an area of 3.32%, and 9-Cedranone constitutes the fifth position in occupying the area percentage [1.88] and height 1.56%. Pyrrole is the sixth primary compound registering an area size of 0.96% and height 2.06%. The AgNPs synthesized using G. lucidum extract were in size range 23 and 58 nm as per SEM analysis and within the range wavelength 0.556–0.796 nm as per UV–Vis spectral study. FTIR Spectroscopy and X-ray diffraction analysis (XRD) were made to characterize the formed nanoparticles. The AgNPs synthesized effectively inhibited the growth of E. coli isolated from catheter-associated urinary tract infection and showed resistance to many drugs. The antioxidant potential of the synthesized nanoparticles assessed using DPPH radical scavenging activity, EC50 (µg/ml), and ARP data showed that the prepared nanoparticles were more potent in free radical scavenging activity than the standard quercetin. The cytotoxicity effect of Ag-NPs on breast cancer cell line- MDA-MB-231 confirmed its anticancer potential. The half-maximal inhibitory concentration (IC₅₀) of Ag-NPs to inhibit 50% of the tumor was 9.2 g/mL. The synthesized GL-AgNPs was exhibited a multifocal biomedical potential.

在寻找感染和其他疾病的替代疗法的过程中，人们广泛探索了通过生物工程合成的金属纳米颗粒，主要是银纳米颗粒（AgNP）。据报道，真菌生物活性化合物及其纳米颗粒具有潜在的生物医学应用。据报道，药用蘑菇灵芝具有丰富的药用特性。在当前的研究中，使用灵芝提取物合成了银纳米粒子，并测试了其对从用于尿路感染的导管（CAUTI）中分离出的耐药大肠杆菌的抗菌活性。灵芝提取物的 GC-MS 研究表明存在乙酰乙酸乙酯乙缩醛，面积百分比最高为 72.2%，保留时间 (RT 5873)。 Pyridine-3-ol 是第二个主要化合物，峰高为 6.44%，保留时间为 2.143。第三个化合物是l,4-二恶烷-2,3-二醇，面积为8.09%，RT 5450。丁基化羟基甲苯[BHT]是第四个主要化合物，面积为3.32%，9-雪松酮构成占据面积百分比[1.88]和高度1.56%排名第五。吡咯是第六种主要化合物，面积大小为 0.96%，高度为 2.06%。根据 SEM 分析，使用灵芝提取物合成的 AgNP 的尺寸范围为 23 和 58 nm，根据 UV-Vis 光谱研究，波长范围为 0.556–0.796 nm。采用 FTIR 光谱和 X 射线衍射分析 (XRD) 来表征所形成的纳米颗粒。合成的AgNPs有效抑制了大肠杆菌的生长。 从导管相关尿路感染中分离出的大肠杆菌对许多药物表现出耐药性。使用 DPPH 自由基清除活性、EC50 (μg/ml) 和 ARP 数据评估合成纳米颗粒的抗氧化潜力，表明所制备的纳米颗粒比标准槲皮素具有更有效的自由基清除活性。 Ag-NPs 对乳腺癌细胞系 MDA-MB-231 的细胞毒性作用证实了其抗癌潜力。 Ag-NPs抑制50%肿瘤的半数最大抑制浓度(IC ₅₀ )为9.2 g/mL。合成的 GL-AgNPs 表现出多焦点生物医学潜力。