In order to explore the function of vitamin C (VC) and VC-Na in the relief of heat stress injury in chicken cardiomyocytes, 150 30-day-old specific-pathogen-free chickens were randomly divided into a control group (fed normal drinking water), a VC group (50 μg/mL VC in drinking water), and a VC-Na group (50 μg/mL VC-Na in drinking water). After 7 days of adaptation feeding, the chickens were subjected to heat stress at 40 ± 2 °C and 60%–70% humidity for 0, 1, 3, 5, and 10 h, respectively, and the sera and heart tissues of the chickens were collected immediately at the corresponding heat stress time points. The effects of VC and VC-Na supplementation on the relief of chicken myocardial cell injury following heat stress was studied by detecting the levels of LDH, CK, CK-MB, and total antioxidant capacity (T-AOC) in the sera, and through histopathological analysis and the expression of CRYAB, Hsp27, and Hsp70 in the myocardial cells. The results showed that supplementing with 50 μg/mL VC or VC-Na significantly reduced the levels of LDH, and CK-MB in serum as well as heat-stress-induced granular and vacuolar degeneration, myocardial fiber breakage, and cell necrosis, indicating effective resistance to heat-stress damage. Additionally, the levels of T-AOC in serum were increased in the VC and VC-Na groups, suggesting enhancing of antioxidant capacity. Furthermore, the expression of CRYAB were induced at 0, 3, 5, and 10 h (P < 0.01) in both VC and VC-Na group, and that of Hsp70 were induced at 0 h (P < 0.05) in VC group and at 0, 3, 5, 10 h (P < 0.01) in VC-Na group. Thus, supplementing chicken diets with VC or VC-Na presented heat-stress damage resistance by enhancing antioxidant capacity and inducing expression of CRYAB and Hsp70.

为了探讨维生素C（VC）和VC-Na在缓解鸡心肌细胞热应激损伤中的作用，将150只30天龄无特定病原体的鸡随机分为对照组（喂食正常饮水水），VC组（饮用水中VC-50μg/ mL）和VC-Na组（饮用水中VC-Na 50μg/ mL）。适应性喂养7天后，将鸡分别在40±2°C和60％–70％的湿度下分别承受0、1、3、5和10 h的热应激，并对其血清和心脏组织进行加热。立即在相应的热应激时间点收集鸡只。通过检测血清中LDH，CK，CK-MB和总抗氧化能力（T-AOC）的含量，研究了VC和VC-Na补充对缓解热应激后鸡心肌细胞损伤的影响，并通过组织病理学分析以及心肌细胞中CRYAB，Hsp27和Hsp70的表达。结果表明，补充50μg/ mL VC或VC-Na可以显着降低血清中LDH和CK-MB的水平，以及热应激诱导的颗粒和液泡变性，心肌纤维断裂和细胞坏死，表明有效抵抗热应力破坏。此外，VC组和VC-Na组的血清中T-AOC含量增加，表明抗氧化能力增强。此外，在0、3、5和10 h诱导了CRYAB的表达（和血清中的CK-MB以及热应激诱导的颗粒和液泡变性，心肌纤维断裂和细胞坏死，表明对热应激损害具有有效的抵抗力。此外，VC组和VC-Na组的血清中T-AOC含量增加，表明抗氧化能力增强。此外，在0、3、5和10 h诱导了CRYAB的表达（和血清中的CK-MB以及热应激诱导的颗粒和液泡变性，心肌纤维断裂和细胞坏死，表明对热应激损害具有有效的抵抗力。此外，VC组和VC-Na组的血清中T-AOC含量增加，表明抗氧化能力增强。此外，在0、3、5和10 h诱导了CRYAB的表达（ VC组和VC-Na组均P <0.01），VC组分别在0 h（P  <0.05）和VC-Na组0、3、5、10 h诱导Hsp70 （P  <0.01） 。因此，在鸡肉中添加VC或VC-Na可以增强抗氧化能力并诱导CRYAB和Hsp70的表达，从而具有抗热应激的作用。