Liver disease has been reported to associate with oral microbiota. This study aimed to identify the salivary microbial structure in liver disease patients and determine whether the disease progression influence the bacterial composition. 16S rDNA high-throughput sequencing and bioinformatic analysis were used to examine oral bacterial diversity in the different status of hepatitis patients including 6 patients with Hepatitis B (Y), 6 patients with Hepatitis B Cirrhosis (YY) and 6 patients with liver cancer (C), and 6 healthy controls (T). Phylogenetic analysis revealed that the genera of Streptococcus, Prevotella, Actinomyces, Veillonella and Neisseria are predominant genus in the saliva of Y, YY, C patients and T group. Lautropia, Abiotrophia and Veillonella were enriched in Y patients, while Treponema, Selenomonas and Oribacterium were also existed in YY patients. Haemophilus, Porphyromonas and Filifactor had high abundance in C patients. The genera of Moryella, Leptotrichia, Lactobacillus, Dialister, Serratia, Enterococcus and Actinobacillus were decreased in all patient samples compared with healthy control samples which may be used for treatment of liver disease. Diversity analyses showed decreased diversity of salivary bacterial communities was discovered in the progress of the liver disease. These findings identified the oral microbiota dysbiosis in liver disease, which may providing available information and possible diagnostic biomarkers for liver patients.

据报道，肝脏疾病与口腔微生物群有关。这项研究旨在鉴定肝病患者的唾液微生物结构，并确定疾病进展是否影响细菌组成。使用16S rDNA高通量测序和生物信息学分析来检查肝炎患者不同状态下的口腔细菌多样性，包括6例乙型肝炎（Y），6例乙型肝炎肝硬化（YY）和6例肝癌（C ）和6个健康对照（T）。系统发育分析表明，链球菌，普氏杆菌，放线菌，Veillonella和Neisseria属。是Y，YY，C和T组患者唾液中的主要属。Lautropia，Abiotrophia和韦荣球菌Y中患者富集，而梅毒螺旋体，月形和Oribacterium在YY患者也存在。丙型肝炎患者的嗜血杆菌，卟啉单胞菌和丝虫具有很高的丰度。的属Moryella，纤毛，乳杆菌，Dialister，沙雷氏菌，肠球菌和放线与可用于治疗肝病的健康对照样品相比，所有患者样品中的血药浓度均降低。多样性分析表明，随着肝脏疾病的进展，唾液细菌群落的多样性下降。这些发现确定了肝病中的口腔微生物群失调，这可能为肝病患者提供可用的信息和可能的诊断性生物标志物。