Traditional Chinese medicine therapy, which can serve as adjuvant therapy for cancer treatment, has no obvious side effects on the human body. Geniposide (GEN), one of the main iridoid glycosides in gardenia fruit, has been widely reported to have anti-cancer effects. In this study, we aimed to inspect whether GEN could inhibit proliferation and promote the apoptosis of human breast cancer cells (MCF-7). In order to better predict the efficacy of GEN, we have prepared the Cs/Gel composite scaffolds by 3D printing technology to mimic the MCF-7 cell growth microenvironment. The prepared Cs/Gel scaffold has good mechanical properties and biocompatibility, which can provide a more accurate platform for drug screening. The semi-inhibitory concentration (IC50) evaluated by CCK-8 assay was 16.06 mg/mL (24 h), 14.85 mg/mL (48 h), and 13.14 mg/mL (72 h). After exposed to GEN for 48h, the cancer cell survival rate reduced from 69.15 ± 2.86% (13 mg/mL) to 20.97 ± 3.24% (16 mg/mL). Although the inhibitory effect was weaker in the 3D culture system, it also managed to inhibit cell proliferation and induce cell apoptosis. Besides, Live/Dead staining, Hematoxylin-Eosin (H&E) staining and SEM evaluation were also conducted to estimate the anti-cancer effect of GEN in 2D and 3D cultures. The results indicate that GEN has an anti-cancer effect based on a time- and dose-dependent manner.

中药疗法可以作为癌症的辅助疗法，对人体没有明显的副作用。ip子苷（GEN）是garden子果实中的主要环烯醚菊糖苷之一，据广泛报道具有抗癌作用。在这项研究中，我们旨在检查GEN是否可以抑制人乳腺癌细胞（MCF-7）的增殖并促进其凋亡。为了更好地预测GEN的功效，我们通过3D打印技术制备了Cs / Gel复合支架，以模拟MCF-7细胞生长的微环境。制备的Cs / Gel支架具有良好的机械性能和生物相容性，可以为药物筛选提供更准确的平台。通过CCK-8分析评估的半抑制浓度（IC50）为16.06 mg / mL（24 h），14.85 mg / mL（48 h）和13.14 mg / mL（72 h）。暴露于GEN 48h后，癌细胞存活率从69.15±2.86％（13 mg / mL）降低至20.97±3.24％（16 mg / mL）。尽管在3D培养系统中抑制作用较弱，但它也能抑制细胞增殖并诱导细胞凋亡。此外，还进行了活/死染色，苏木精-伊红（H＆E）染色和SEM评价，以评估GEN在2D和3D培养物中的抗癌作用。结果表明，GEN具有基于时间和剂量依赖性的抗癌作用。E）还进行染色和SEM评估以估计GEN在2D和3D培养物中的抗癌作用。结果表明，GEN具有基于时间和剂量依赖性的抗癌作用。E）还进行染色和SEM评估以估计GEN在2D和3D培养物中的抗癌作用。结果表明，GEN具有基于时间和剂量依赖性的抗癌作用。