Rat models of duodenogastric reflux have been used to study gastric stump cancer (GSC), but the underlying molecular mechanisms are poorly understood. Unlike rats, mice can be genetically modified, providing a superior model for studying the molecular mechanisms underlying GSC development, which is associated with duodenogastric reflux. This study aimed at developing a mouse model of duodenogastric reflux. C57BL/6 mice were randomly assigned to the control ( = 6), sham operation ( = 9), or gastrojejunostomy group ( = 12). Mice were sacrificed at 1, 3, and 6 months after surgery. Stomach tissue was stained with hematoxylin and eosin. Lesions were classified as chronic inflammation, intestinal metaplasia, or atypical hyperplasia. Nine mice underwent gastrojejunostomy without mortality. The animals in the gastrojejunostomy group exhibited chronic inflammation at 1, 3, and 6 months after surgery, showing intestinal metaplasia ( = 2) and atypical hyperplasia ( = 1) at 3 months and intestinal metaplasia (n = 2) and atypical hyperplasia (n = 2) at 6 months. The mice in the control group did not exhibit chronic inflammation or intestinal metaplasia, whereas those in the sham operation group exhibited chronic inflammation at 1, 3, and 6 months after surgery, without intestinal metaplasia or atypical hyperplasia. Intestinal metaplasia or atypical hyperplasia were more common in the gastrojejunostomy group than in the sham operation group ( = 0.012). A duodenogastric reflux mouse model can be created using gastrojejunostomy without gastrectomy.

中文翻译：

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十二指肠胃反流小鼠模型的开发和表征


十二指肠胃反流的大鼠模型已被用于研究残胃癌（GSC），但其潜在的分子机制尚不清楚。与大鼠不同，小鼠可以进行基因改造，为研究 GSC 发育的分子机制（与十二指肠胃反流相关）提供了一个优越的模型。本研究旨在开发十二指肠胃反流的小鼠模型。 C57BL/6 小鼠被随机分配到对照组 (= 6)、假手术组 (= 9) 或胃空肠吻合术组 (= 12)。手术后 1、3 和 6 个月处死小鼠。胃组织用苏木精和曙红染色。病变分为慢性炎症、肠化生或不典型增生。九只小鼠接受了胃空肠造口术，没有死亡。胃空肠吻合组动物在术后1、3、6个月表现出慢性炎症，3个月时表现出肠化生（n = 2）和不典型增生（n = 1），以及肠化生（n = 2）和不典型增生（n）。 = 2) 6 个月时。对照组小鼠未表现出慢性炎症或肠化生，而假手术组小鼠在术后1、3和6个月时表现出慢性炎症，但未出现肠化生或不典型增生。胃空肠吻合组肠化生或不典型增生比假手术组更常见（= 0.012）。可以使用胃空肠吻合术创建十二指肠胃反流小鼠模型，而无需进行胃切除术。