N-glycosylation profiling of serum immunoglobulin in opisthorchiasis patients.,Journal of Proteomics

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N-glycosylation profiling of serum immunoglobulin in opisthorchiasis patients.
Journal of Proteomics ( IF 2.8 ) Pub Date : 2020-09-11 , DOI: 10.1016/j.jprot.2020.103980
Sudarat Onsurathum ₁ , Muchena J Kailemia ₂ , Kitti Intuyod ₃ , Ornuma Haonon ₃ , Chawalit Pairojkul ₄ , Raynoo Thanan ₅ , Porntip Pinlaor ₆ , Carlito B Lebrilla ₂ , Somchai Pinlaor ₃

Affiliation

Alteration of immunoglobulin glycosylation correlates with inflammatory diseases and infectious diseases including parasitic infections. Immunoglobulin glycosylation patterns may be implicated in disease development and have also been proposed as diagnostic tools for several diseases. Previous studies have reported the immunoglobulin profiles in experimental animals and in patients infected with the carcinogenic human liver fluke, Opisthorchis viverrini. However, the N-glycosylation profiles of immunoglobulins and their subclass-specific glycoforms in opisthorchiasis patients have never been elucidated. Here, N-glycosylation patterns of immunoglobulins and their subclass-specific glycoforms in sera of O. viverrini-infected patients were investigated using triple quadrupole mass spectrometry coupled with multiple reaction monitoring. Peptide fragmentation was utilized to quantify the immunoglobulin glycoforms normalized to the unique peptide of each subclass. Overall, serum levels of IgG and IgA in O. viverrini patients were significantly increased compared to uninfected controls. Twenty-seven glycoforms were detected based on analysis of detached glycans in all immunoglobulin subclasses. The abundance of immunoglobulin glycopeptides in serum of opisthorchiasis patients deviated significantly from controls. Immunoglobulin glycosylation patterns were associated with both pro- and anti-inflammatory properties. In conclusion, O. viverrini infection alters the serum immunoglobulin glycosylation profile and these changes could distinguish between O. viverrini-infected individuals and healthy controls.

Significance

We demonstrated that both quantities and glycoforms of serum immunoglobulin subclasses were altered in Opisthorchis viverrini-infected individuals as investigated by the QqQ-MS-MRM method. Patterns of immunoglobulin with a specific glycoform might contribute to immune responses to O. viverrini infection.

中文翻译：

阿司匹林患者血清免疫球蛋白的N-糖基化分析。

免疫球蛋白糖基化的改变与炎性疾病和包括寄生虫感染在内的传染病有关。免疫球蛋白糖基化模式可能与疾病发展有关，也已被提议作为多种疾病的诊断工具。先前的研究报道了实验动物和被致癌性人类肝吸虫Opisthorchis viverrini感染的患者的免疫球蛋白谱。然而，尚未阐明免疫球蛋白及其亚类特异性糖型的N-糖基化谱。这里，Ñ -glycosylation免疫球蛋白的图案和在血清其亚类特异性的糖形O.吸虫三重四极杆质谱结合多重反应监测对感染患者进行了研究。利用肽片段化来量化针对每个亚类的独特肽标准化的免疫球蛋白糖型。总体而言，与未感染的对照组相比，维氏曲霉患者的血清IgG和IgA水平显着增加。根据对所有免疫球蛋白亚类中分离的聚糖的分析，检测到27种糖型。阿斯匹美氏病患者血清中免疫球蛋白糖肽的丰度明显不同于对照组。免疫球蛋白的糖基化模式与促炎和抗炎特性有关。最后，O。viverrini感染改变了血清免疫球蛋白的糖基化谱，这些变化可以区分感染维氏曲霉的个体和健康对照。