The ability to screen larger populations with fewer replicates and non-destructive measurements is one advantage of high-throughput phenotyping (HTP) over traditional phenotyping techniques. In this study, two wheat accessions were grown in a controlled-environment with a moderate drought imposed from stem elongation to post-anthesis. Red-green-blue (RGB) imaging was performed on 17 of the 22 d following the start of drought imposition. Destructive measurements from all plants were performed at the conclusion of the experiment. The effect of line was significant for shoot dry matter, spike dry matter, root dry matter, and tiller number, while the water treatment was significant on shoot dry matter and root dry matter. The temporal, non-destructive nature of HTP allowed the drought treatment to be significantly differentiated from the well-watered treatment after 6 d in a line from Argentina and 9 d in a line from Chile. This difference of 3 d indicated an increased degree of drought tolerance in the line from Chile. Furthermore, HTP from the final day of imaging accurately predicted reference plant height (r=1), shoot dry matter (r=0.95) and tiller number (r=0.91). This experiment illustrates the potential of HTP and its use in modeling plant growth and development.

与传统表型技术相比，高通量表型（HTP）的优势之一是能够以较少的重复和无损测量来筛查更大的种群。在这项研究中，两个小麦种质均在受控环境中生长，从茎伸长到花后后期出现中等干旱。施加干旱后，在22天中的17天中进行了红绿蓝（RGB）成像。在实验结束时对所有植物进行破坏性测量。线对茎干物质，穗干物质，根系干物质和分till数的影响显着，而水处理对茎干物质和根系干物质的影响显着。暂时的 HTP的无损性质使干旱处理与阿根廷生产线中的6天和智利生产线中的9天后与干旱灌溉有明显区别。3 d的差异表明来自智利的品系的耐旱性提高。此外，从成像的最后一天开始的HTP可以准确地预测参考植物高度（r = 1），枝干物质（r = 0.95）和分er数（r = 0.91）。该实验说明了HTP的潜力及其在模拟植物生长和发育中的用途。95）和分er号（r = 0.91）。该实验说明了HTP的潜力及其在模拟植物生长和发育中的用途。95）和分er号（r = 0.91）。该实验说明了HTP的潜力及其在模拟植物生长和发育中的用途。