Depending on the coordination, copper ions can have a very high activity in catalyzing the production of reactive oxygen species. Thus interest arose in increasing the activity of antimicrobial peptides (AMPs) by equipping them with a Cu-binding unit. Several examples, native and engineered, have been investigated with the motif Xxx-Zzz-His, called Amino Terminal Cu(II)- and Ni(II)-binding (ATCUN) motif. Here we investigate a short AMP that was equipped either with Xxx-Zzz-His or Xxx-His. Xxx-His is a shorter motif and yields a more redox active copper complex. The control AMP, Xxx-His-AMP and Xxx-Zzz-His-AMP were investigated toward Cu-binding, Reactive Oxygen Species (ROS) production and antimicrobial activity in E. coli. The data indicate that these Cu-binding motifs have very limited impact on antimicrobial activity and low ROS production capability.

取决于配位，铜离子在催化活性氧的产生中可以具有非常高的活性。因此，通过为它们配备一个Cu结合单元来增加其抗菌肽的活性引起了人们的兴趣。已经研究了一些名为Xxx-Zzz-His的天然和工程实例，称为氨基末端Cu（II）-和Ni（II）-结合（ATCUN）基序。在这里，我们研究了装有Xxx-Zzz-His或Xxx-His的短AMP。Xxx-His是一个较短的基序，并产生更多的氧化还原活性铜络合物。研究了对照AMP，Xxx-His-AMP和Xxx-Zzz-His-AMP在大肠杆菌中的Cu结合，活性氧（ROS）产生和抗菌活性。数据表明，这些铜结合基序对抗菌活性和低ROS生产能力的影响非常有限。