Francisella noatunensis subsp. noatunensis is the responsible agent of Francisellosis, a bacterial disease that affects an important amount of aquatic farmed species. Eleginops maclovinus is a fish that cohabits with salmonids cages in Chile and can also act as a vector of this bacterial disease. In the present study, we evaluated calcium metabolism in the liver of E. maclovinus injected intraperitoneally with different doses of F. noatunensis subsp. noatunensis (low 1.5 × 10¹, medium 1.5 × 10⁵ and high doses 1.5 × 10¹⁰ cells/μL). Fish were sampled at 1, 3, 7, 14, 21 and 28 days post injection (dpi). No mortalities nor clinical signs were observed. Plasma calcium levels were higher in the high doses group of F. noatunensis subsp. noatunensis at day 7 and 14 compared to the control group (fish injected with bacterial medium alone). Hypercalcemic factors increased at day 14 and 21 for the medium and low dose (parathyroid hormone-related protein precursor), while vitamin D₃ receptor increased its expression at times 1, 3 and 7 for the low dose. On the other hand, hypocalcemic factors such as calcitonin receptor and stanniocalcin increased its expression at time 7 and 14, respectively. Calmodulin involved in calcium storage decreased its expression during all experimental days in fish subjected to high bacterial dose. Proteins involved in calcium transport, such as L-type voltage-gated calcium channel and trpv5 increased their transcription at day 1 and 14, compared to calcium sensing-receptor and plasma membrane Ca^{2 +}- ATPase that showed peak expression at times 14 and 28. The results suggest a clear alteration of calcium metabolism, mainly in high bacterial doses. This study provides new knowledge about the calcium metabolism in fish infected with bacteria.

弗朗西斯菌noatunensis亚种。Noatunensis是Francisellosis的病原体，Franciscellosis是一种细菌性疾病，会影响大量的水生养殖物种。Eleginops maclovinus是一种鱼，与智利的鲑鱼笼共栖，也可以作为这种细菌性疾病的媒介。在本研究中，我们评估了腹膜内注射不同剂量的No.unatunensis亚种的Maclovinus肝脏中的钙代谢。noatunensis（低1.5×10 ¹，中1.5×10 ⁵和高剂量1.5×10 ¹⁰细胞/微升）。在注射后第1、3、7、14、21和28天（dpi）对鱼取样。没有观察到死亡或临床症状。高剂量F. noatunensis亚种的血浆钙水平较高。与对照组相比，第7天和第14天的Noatunensis（仅注射细菌培养基的鱼）。中，低剂量（甲状旁腺激素相关蛋白前体）的第14天和第21天高钙血症因子增加，而维生素D ₃低剂量时，受体在时间1、3和7时表达增加。另一方面，降血钙因子如降钙素受体和锡降钙素分别在时间7和14时增加其表达。在所有实验日中，参与高细菌剂量的鱼中参与钙储存的钙调蛋白降低了其表达。与钙敏感受体和质膜Ca ^{2 +}相比，参与钙转运的蛋白质（例如L型电压门控钙通道和trpv5）在第1天和第14天增加了它们的转录。-ATP酶在14和28时表达高峰。结果表明钙代谢发生明显变化，主要是在高细菌剂量下。这项研究提供了有关被细菌感染的鱼类中钙代谢的新知识。