Our recent report demonstrated that hesperetin (Hst) as a citrus flavonoid, significantly reduces the levels of demyelination in optic chiasm of rats. Previous evidence also indicated that nano-hesperetin (nano-Hst) possesses beneficial impacts in experimental models of Alzheimer's disease and autism. In this study, the effects of nano-Hst on latency of visual signals, demyelination levels, glial activation, and expression of Olig2 and MBP were evaluated in lysolecithin (LPC)-induced demyelination model. Focal demyelination was induced by injection of LPC (1%, 2 μL) into the rat optic chiasm. Animals received oral administration of nano-Hst at dose of 20 mg/kg for 14 or 21 days post LPC injection. Visual evoked potential (VEP) recording showed that nano-Hst reduces the latency of visual signals and ameliorates the extent of demyelination areas and glial activation. Expression levels of the Olig2 and MBP were also significantly increased in nano-Hst treated rats. Overall, our data suggest that nano-Hst reduces the latency of visual signals through its protective effects on myelin sheath, amelioration of glial activation, and enhancement of endogenous remyelination.

我们最近的报告表明橙皮素 (Hst) 作为一种柑橘类黄酮，可显着降低大鼠视交叉的脱髓鞘水平。先前的证据还表明，纳米橙皮素 (nano-Hst) 对阿尔茨海默病和自闭症的实验模型具有有益影响。在本研究中，nano-Hst 对视觉信号延迟、脱髓鞘水平、神经胶质激活以及Olig2和MBP表达的影响在溶血卵磷脂（LPC）诱导的脱髓鞘模型中进行了评估。通过将 LPC（1%，2 μL）注射到大鼠视交叉中来诱导局灶性脱髓鞘。在LPC注射后14或21天，动物以20mg/kg的剂量接受nano-Hst的口服给药。视觉诱发电位 (VEP) 记录显示纳米 Hst 减少了视觉信号的延迟并改善了脱髓鞘区域和神经胶质激活的程度。Olig2和MBP 的表达水平在 nano-Hst 处理的大鼠中也显着增加。总体而言，我们的数据表明，nano-Hst 通过其对髓鞘的保护作用、改善神经胶质激活和增强内源性髓鞘再生来减少视觉信号的延迟。