The objective of the present work was the selection of cultivar, suitable medium and explant type for callus, root production, ascorbic acid, total ascorbic acid, dehydroascorbic and total protein of non-heading Chinese cabbage in two cultivars ‘Caixin’ and ‘Suzhouqing’. We compared 10 types of MS media supplemented with 0.0, 1.0, 2.0 and 3.0 mg/l TDZ; 0.0, 0.25, 0.50 and 1.0 mg/l NAA and 0.0, 5.0, 7.5 and 9.0 mg/l AgNO₃ and 5 kinds of explants as embryo, leaf, root, cotyledon and hypocotyl. Maximum frequency of callus fresh weight was recorded with hypocotyl explant, which were cultured on MS + 2.0 mg/l TDZ + 1.0 mg/l NAA + 9.0 mg/l AgNO₃ in ‘Suzhouqing’, optimum callus dry weight was obtained on the same media. The highest result for root fresh and dry weight recorded with ‘Caixin’ with MS + 3.0 mg/l TDZ + 1.0 mg/l NAA + 9.0 mg/l AgNO₃ when we used embryo as explant. The highest ascorbic acid content was found with callus cultured on MS + 1.0 mg/l TDZ + 0.25 mg/l NAA + 5.0 mg/l AgNO₃, when used leaf explant in ‘Caixin’ or root in ‘Suzhouqing’, and there were no significant difference between them. While the highest value of total AsA content was registered with callus cultured on MS + 2.0 mg/l TDZ + 0.25 mg/l NAA + 5.0 mg/l AgNO₃ extracted from cotyledon in ‘Caixin’. The highest content of DHA was registered with MS + 2.0 mg/l TDZ + 0.25 mg/l NAA + 5.0 mg/l AgNO₃ with cotyledon in ‘Caixin’. Also, in ‘Caixin’ MS + 3.0 mg/l TDZ + 0.25 mg/l NAA + 5.0 mg/l AgNO₃ recorded the highest value of total protein content with embryo explant.

本工作的目的是选择两个品种'菜心'和'苏州青'的愈伤组织，根生，抗坏血酸，总抗坏血酸，脱氢抗坏血酸和无protein大白菜总蛋白的品种，合适的培养基和外植体类型。 。我们比较了添加0.0、1.0、2.0和3.0 mg / l TDZ的10种MS培养基；0.0、0.25、0.50和1.0 mg / l NAA以及0.0、5.0、7.5和9.0 mg / l AgNO ₃和5种外植体，如胚芽，叶，根，子叶和下胚轴。用胚轴外植体记录愈伤组织鲜重的最大频率，在MS + 2.0 mg / l TDZ + 1.0 mg / l NAA + 9.0 mg / l AgNO ₃上培养在“苏州青”中，在相同培养基上获得了最佳的愈伤组织干重。当我们将胚用作外植体时，用“菜心素”以MS + 3.0 mg / l TDZ + 1.0 mg / l NAA + 9.0 mg / l AgNO ₃记录的最高根鲜重和干重结果。当在'菜心'或'苏州青'的根部上进行叶移植时，用MS + 1.0 mg / l TDZ + 0.25 mg / l NAA + 5.0 mg / l AgNO ₃培养的愈伤组织发现抗坏血酸含量最高。它们之间没有显着差异。从“菜心”中从子叶中提取的MS + 2.0 mg / l TDZ + 0.25 mg / l NAA + 5.0 mg / l AgNO ₃上培养的愈伤组织记录了总AsA含量的最高值。DHA含量最高的是MS + 2.0 mg / l TDZ + 0.25 mg / l NAA + 5.0 mg / l AgNO ₃与子叶在“菜心”中。同样，在“菜芯”中，MS + 3.0 mg / l TDZ + 0.25 mg / l NAA + 5.0 mg / l AgNO ₃记录了胚胎外植体中总蛋白含量的最高值。