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Complex Analysis of Fluorescence Intensity Fluctuations of Molecular Compounds
Journal of Applied Spectroscopy ( IF 0.8 ) Pub Date : 2020-09-11 , DOI: 10.1007/s10812-020-01055-6
M. M. Yatskou , V. V. Skakun , L. Nederveen-Schippers , A. Kortholt , V. V. Apanasovich

A method is proposed for the complex analysis of fluctuations in the fluorescence intensity of molecular compounds, which allows determining the structural composition of protein oligomers. The idea of the method is to analyze the photon counting histograms of experimental measurements using principal component analysis to assess the presence of oligomeric compounds, and to perform hierarchical cluster analysis, to determine the data classes corresponding to various molecular compounds, followed by selecting cluster medoids to determine the oligomeric composition of protein complexes. The efficiency of the analysis algorithms developed within the framework of the proposed method was confirmed on simulated and experimental photon counting histograms of the measured fluorescence intensity fluctuations of monomeric and dimeric forms of green-fluorescent protein (GFP).



中文翻译:

分子化合物荧光强度波动的复杂分析

提出了一种对分子化合物的荧光强度波动进行复杂分析的方法,该方法可以确定蛋白质低聚物的结构组成。该方法的思想是使用主成分分析来分析实验测量的光子计数直方图,以评估低聚化合物的存在,并进行层次聚类分析,以确定与各种分子化合物相对应的数据类别,然后选择聚簇类固醇确定蛋白质复合物的寡聚组成。

更新日期:2020-09-11
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