We investigated the effect of drugs (dapsone, paracetamol, and isoniazid) on the production of reactive oxygen and halogen species by neutrophils. The standard fluorescent method using scopoletin as well as recently developed fluorescent methods using oxazine dyes, celestine blue B, and gallocyanine were employed for this purpose. Celestine blue B reacts selectively with hypochlorous acid, while gallocyanine reacts mainly with the superoxide radical-anion, which reveals the regulatory effect of anti-inflammatory drugs on neutrophil NADPH-oxidase and myeloperoxidase responsible for the production of reactive oxygen and halogen species, respectively. These results indicate that gallocyanine and celestine blue B hold promise as chemosensors for studying the effect of drugs used in anti-inflammatory therapy for the neutrophil respiratory burst.

我们研究了药物（氨苯砜，扑热息痛和异烟肼）对中性粒细胞产生活性氧和卤素的影响。为此目的，采用了使用草素的标准荧光方法以及最近开发的使用恶嗪染料，天青蓝B和没食子花青的荧光方法。Celestine blue B与次氯酸选择性反应，而gallocyanine主要与超氧自由基阴离子反应，这揭示了消炎药对中性粒细胞NADPH氧化酶和髓过氧化物酶的调节作用，分别负责产生活性氧和卤素。这些结果表明，关于研究嗜中性粒细胞呼吸爆发的消炎药所用药物的作用，没食子花青素和蔚蓝B具有广阔的前景。