In vitro culture is employed as a means of producing plant materials from medicinal plants to meet the increasing demands of the market. Herein, we focused on an efficient protocol for organogenesis through two pathways in six explant types from Caryopteris terniflora Maxim (C. terniflora), a medicinal and ornamental plant. For direct organogenesis, the maximum number of shoots per internode explant (62.5) was achieved on Murashige and Skoog (MS) medium containing 3.0 mg L⁻¹ thidiazuron (TDZ) and the maximum number of shoot tips per explant (45.5) was obtained by 1.5 mg L⁻¹ TDZ, with 100% response. A protocol was also developed for indirect organogenesis from leaf, leaf-petiole, petiole, and root explants on MS basal medium containing a range of TDZ concentrations. Leaf-petiole explants induced more adventitious shoots (57.6 per explant) and leaves (31.4 per explant) than petioles (37.5 per explant) or roots (25.0 per explant), and the differentiation rate ranged from 87.2 to 100%. The regenerated shoots were elongated on MS medium containing 0.3 mg L⁻¹α-naphthaleneacetic acid (NAA) and 1.0 mg L⁻¹ gibberellic acid (GA₃). The elongated shoots developed roots on ½ MS medium containing 0.3 mg L⁻¹ NAA. Successful acclimatization of rooted plantlets in a greenhouse was achieved with 100% survival. Furthermore, the embryogenic-like structure was observed and the morphological characteristics of C. terniflora assessed at different stages of in vitro development. Our protocol offers, for the first time, an efficient, scalable propagation procedure for C. terniflora useful for large-scale cultivation for the purposes of conservation and genetic manipulation.

体外培养被用作从药用植物生产植物材料的手段，以满足日益增长的市场需求。在这里，我们集中于通过药用和观赏植物Caryopteris terniflora Maxim（C. terniflora）的六种外植体类型中的两种途径进行器官发生的有效方案。对于直接的器官发生，在含有3.0 mg L ^-1噻唑隆（TDZ）的Murashige和Skoog（MS）培养基上达到了每个节间外植体的最大芽数（62.5），并且通过以下方法获得了每个外植体的最大芽尖数（45.5）： 1.5毫克L ^-1TDZ，具有100％的响应。还开发了一种协议，用于在含有一定范围TDZ浓度的MS基础培养基上从叶，叶柄，叶柄和根外植体进行间接器官发生。叶柄外植体比叶柄（每株37.5）或根（每株25.0）诱导不定芽（每株57.6）和叶片（每株31.4）或根，分化率在87.2％至100％之间。将再生的芽在含有0.3mg L ^-1α-萘乙酸（NAA）和1.0mg L ^-1赤霉素（GA ₃）的MS培养基上伸长。伸长的枝条在含有0.3 mg L ^-1的½MS培养基上生长出根NAA。在温室中成功实现了生根小苗的驯化，存活率为100％。此外，观察到了胚发生样结构，并在体外发育的不同阶段评估了C. terniflora的形态特征。我们的协议首次提供了一种有效的，可扩展的菊苣花繁殖程序，可用于大规模栽培，以用于保存和遗传操作。