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Reaction of clubroot-resistant genotypes of Brassica rapa, Brassica napus and Brassica oleracea to Polish Plasmodiophora brassicae pathotypes in laboratory tests
European Journal of Plant Pathology ( IF 1.7 ) Pub Date : 2020-08-26 , DOI: 10.1007/s10658-020-02100-y
Agnieszka Czajka , Monika Markiewicz , Beata Kowalska , Urszula Smolińska

The Brassica genotypes selected for the experiments were previously found to be resistant to various Plasmodiophora brassicae pathotypes (Pb). Their interaction with pathotypes Pb2, Pb3 and Pb9 isolated in Poland was studied, using macroscopic observation for the presence of root galls, microscopic observations of P. brassicae plasmodia in the root hairs and quantitative PCR for determination of the pathogen’s quantity in plant roots and growing media. Of the Brassica genotypes studied, only B. rapa var. capitata line AABBcc was fully resistant to all the Polish pathotypes of P. brassicae. Some of the other “clubroot-resistant” genotypes tested were resistant to selected pathotypes, e.g. Brassica napus var. rapifera ‘Wilhelmsburger’ to Pb 2 and Pb3, Brassica oleracea var. capitata ‘Kilaton F1’ to Pb2 and Brassica rapa subsp. pekinensis ‘Bilko F1’ to Pb3, but were susceptible to others. B. oleracea var. capitata ‘Bindsachsener’, B. oleracea var. acephala subvar. lacinata ‘Verheul’ and B. napus var. napus ‘Mendel F1’ were moderately to highly susceptible to all Polish P. brassicae pathotypes. These results show that the classification of virulence of P. brassicae pathotypes selected in various areas differs significantly toward the same Brasssica genotypes and puts in question the practical value of pathotype classification determined with differential sets for farmers and plant breeders. Our results showed that B. rapa var. capitata AABBcc line, B. napus var. rapifera ‘Wilhelmsburger’, B. oleracea var. capitata ‘Kilaton F1, B. rapa subsp. pekinensis ‘Bilko F1’ could be considered in Brassica breeding programmes as a source of resistance to Polish P. brassicae isolates.



中文翻译:

在实验室测试中,甘蓝型油菜,甘蓝型油菜和甘蓝型油菜根茎抗性基因型对波兰小菜蛾的反应型的反应

先前发现,为实验选择的芸苔属基因型对各种芸苔疟原虫致病型(Pb)具有抗性。研究了它们与波兰分离的致病型Pb2,Pb3和Pb9的相互作用,使用肉眼观察了根gall的存在,用显微镜观察了根毛中的芸苔疟原虫,并通过定量PCR确定了植物根和生长中的病原体数量。媒体。在研究的芸苔属基因型中,只有B. rapa var。capitata品系AABBcc对所有的波兰芸苔假单胞菌均具有完全抗性测试的其他一些“抗clubroot”基因型对选定的病态具有抗性,例如甘蓝型油菜rapifera'Wilhelmsburger '到Pb 2和Pb3,甘蓝变种。实蝇“Kilaton˚F 1 ”到PB2和芜菁亚种。pekinensis'Bilko F 1 '到Pb3,但容易被他人感染。油菜变种 capitata'Bindsachsener ',oleracea var。变种 lacinata'Verheul '和B. napus var。甘蔗'门德F 1对所有波兰芸苔属病原体均中等至高度敏感。这些结果表明,在不同地区选择的芸苔病原菌毒力分类对相同的芸苔属基因型有显着差异,并质疑用差异集确定的病原菌分类对农民和植物育种者的实用价值。我们的结果表明,B。rapa var。结球甘蓝AABBCC线,甘蓝型油菜变种。rapifera'Wilhelmsburger ',B. oleracea var 。capitata'Kilaton F 1 'B. rapa subsp。pekinensis'Bilko F 1 '在芸苔属育种计划中被认为是对波兰芸苔科分离株的抗性来源。

更新日期:2020-09-11
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