To determine deferasirox in human breast milk a novel, sensitive high-performance liquid chromatographic method with fluorescence detection has been developed. Precolumn derivatization process is applied to achieve fluorescent analyte using 4-bromomethyl-7-methoxy coumarin (BrMmC) as a reagent and dibenzo-18-crown-6-ether as catalyst. The method depends on reverse phase chromatography. C18 column has been used with 150 × 4.6 mm, 5 μm particle size and the mobile phase consisting of acetonitrile:phosphate buffer (20 mM, pH 3) (70:30, v/v) with a flow rate of 1.0 mL/min. Fluorescence detection at 390 nm is provided with an excitation at 320 nm The linear range is 0.01–1.0 μg/mL. The mean recovery has been calculated as 100.7% with a relative standard deviation (RSD) value less than 2%. The developed method was successfully applied for the determination of deferasirox in human breast milk samples.

中文翻译：

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使用荧光检测法对母乳中地拉罗司进行液相色谱分析

为了测定人母乳中的地拉罗司，我们开发了一种新型、灵敏的荧光检测高效液相色谱方法。以4-溴甲基-7-甲氧基香豆素（BrMmC）为试剂，二苯并18-冠-6-醚为催化剂，采用柱前衍生化工艺获得荧光分析物。该方法依赖于反相色谱法。已使用 C18 色谱柱，粒径为 150 × 4.6 mm，5 μm，流动相由乙腈:磷酸盐缓冲液 (20 mM, pH 3) (70:30, v/v) 组成，流速为 1.0 mL/min . 390 nm 荧光检测由 320 nm 激发提供。线性范围为 0.01–1.0 μg/mL。平均回收率计算为 100.7%，相对标准偏差 (RSD) 值小于 2%。