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MRI visualization of shiitake mycelium growing in logs in order to support shiitake mushroom log cultivation
Cellulose ( IF 4.9 ) Pub Date : 2020-09-11 , DOI: 10.1007/s10570-020-03407-z
Kuniyasu Ogawa , Takeshi Yashima

In order to correlate the yield and quality of shiitake mushrooms with the growth process of shiitake mycelium in logs over several years, a non-invasive visualization method using a magnetic resonance imaging (MRI) measurement system was developed. The MRI measurement system had a permanent magnet of 1.0 T magnetic field strength and a 110 mm air gap. The test logs in which grew the shiitake mycelium had a diameter of 43–48 mm, a length of 110–118 mm and a dried mass of 125–135 g. The log was inoculated on one cutting surface after being supplied water, and was placed in a thermostatic chamber (21 °C, about 75% humidity) for 69 days. The test log was observed by MRI, and MR images and T1 maps of shiitake mycelium growing over time were obtained. It was found that the shiitake mycelium has a T1 relaxation time constant longer than 150–200 ms, and its signal intensity in the MR images is smaller than that of water penetrated into a log. When making the MRI measurements, it is necessary to dry a log to water content of about 20% or less. In addition, a control log with mold was also observed by MRI. The shiitake mycelium can be distinguished from water penetrated into log and mold occurring in the log by combining the T1—signal intensity map and clear recognition of a low-intensity region in MR images acquired at longer echo times.



中文翻译:

为了支持香菇原木栽培而在原木中生长的香菇菌丝体的MRI可视化

为了使香菇的产量和品质与香菇菌丝体在数年内的生长过程相关联,开发了一种使用磁共振成像(MRI)测量系统的无创可视化方法。MRI测量系统具有1.0 T磁场强度的永久磁铁和110 mm的气隙。生长香菇菌丝体的原木直径为43-48毫米,长度为110-118毫米,干燥质量为125-135克。供应原木后,将原木接种在一个切割面上,并将其放置在恒温室(21°C,约75%的湿度)中69天。通过MRI观察测试日志,并获得香菇菌丝体随时间生长的MR图像和T 1图。发现香菇菌丝具有T 1弛豫时间常数大于150–200 ms,其MR图像中的信号强度小于渗入测井仪中的水的信号强度。进行MRI测量时,有必要将原木干燥至水分含量不超过20%左右。另外,还通过MRI观察到带有霉菌的对照日志。通过结合T 1-信号强度图和在较长回波时间获得的MR图像中的低强度区域的清晰识别,可以将香菇菌丝体与渗入圆木和渗入圆木中的霉菌的水区分开。

更新日期:2020-09-11
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