The purpose of this study is to enhance the biodegradability of atrazine with FH-1 and NJ-1 alone by selecting the mixing ratio, optimizing the culture medium and conditions. The results showed that FH-1 and NJ-1 have the best biodegradation effect on atrazine being mixed in a volume ratio of 3:2. In a single factor experiment, sucrose and NH₄Cl provided carbon and nitrogen sources for the mixed bacteria. Subsequently, composition of fermentation medium was further optimized using Box-Behnken design of response surface methodology. Based on the results, growth of mixed bacteria and biodegradation of atrazine performed best effects with a biodegradation rate of 85.6% when sucrose and NH₄Cl amounts were 35.30 g/L and 10.28 g/L. The optimal medium condition was 10% inoculum of mixed bacteria, with initial atrazine concentration of 50 mg/L, neutral or weakly alkaline pH value, 30°C. The biodegradation rate reached 97.4%, 11.8% higher than the unoptimized condition.

本研究的目的是通过选择混合比例，优化培养基和条件来增强阿特拉津与FH-1和NJ-1的生物降解能力。结果表明，以3：2的体积比混合FH-1和NJ-1具有最佳的生物降解效果。在单因素实验中，蔗糖和NH ₄ Cl为混合细菌提供了碳和氮源。随后，使用响应面方法的Box-Behnken设计进一步优化了发酵培养基的组成。根据结果​​，蔗糖和NH ₄时，混合细菌的生长和r去津的生物降解效果最佳，生物降解率为85.6％。Cl含量为35.30g / L和10.28g / L。最佳培养基条件是混合菌接种量为10％，初始at去津浓度为50 mg / L，中性或弱碱性pH值为30°C。生物降解率达到97.4％，比未优化条件高11.8％。