During searching for marine endophytic fungi that have the potential to effectively produce lovastatin using agro-industrial residues, the endophytic Fusarium sp. ALAA-20 was isolated from marine sponge Aplysina fistularis with lovastatin production of 4.51 mg/gds in the solid-state fermentation of rice straw. Lovastatin yield was improved by enhancing approaches including mutation induction and three consecutive cycles of genome shuffling of the producing strain ALAA-20 to obtain the hyperactive recombinant strain FR3/1 with a higher yield of lovastatin (52.1 mg/gds), which is 11.55- and 3.1-fold of the parent strain ALAA-20 and the hyperactive mutant NE21 (16.8 mg/gds), respectively. Moreover, optimization of the solid-state fermentation of oil cakes by the recombinant FR3/1 significantly increased the lovastatin production by 64.19-, 17.23- and 5.56-fold over the selected wild strain (ALAA-20), mutant (NE21) and fusant (FR3/1) prior to the optimization process. Lovastatin obtained from the recombinant FR3/1 strain showed pleiotropic effects such as antibacterial activity against drug-resistant Staphylococcus, Pseudomonas and Klebsiella species and antifungal activity against Candida, Aspergillus, Fusarium and Trichophyton species. New recombinant strain FR3/1 lovastatin showed potent antitumor activity against liver (HepG-2), colon (HCT-116), breast (MCF-7) and lung (A-549) cancer cell lines with IC₅₀ equal to 8.0, 7.2, 4.8 and 9.1 μM, respectively.

在寻找有潜力利用农用工业残留物有效生产洛伐他汀的海洋内生真菌时，内生镰刀菌属。从海海绵Aplysina fistularis中分离出ALAA-20稻草固态发酵中洛伐他汀的产量为4.51 mg / gds。通过增强方法，包括突变诱导和生产菌株ALAA-20的三个连续基因组改组循环，可以提高洛伐他汀的产量，从而获得高活性洛伐他汀（52.1 mg / gds）的高活性重组菌株FR3 / 1，即11.55-分别为亲本菌株ALAA-20和高活性突变体NE21（16.8 mg / gds）的3.1倍。此外，通过重组FR3 / 1优化油饼的固态发酵，与所选野生株（ALAA-20），突变体（NE21）和融合蛋白相比，洛伐他汀的产量显着提高了64.19-，17.23-和5.56倍。 （FR3 / 1）在优化过程之前。金黄色葡萄球菌，假单胞菌和克雷伯菌属物种以及对念珠菌，曲霉，镰刀菌和毛癣菌物种的抗真菌活性。新的重组菌株FR3 / 1洛伐他汀显示对肝（HepG-2），结肠（HCT-116），乳腺癌（MCF-7）和肺癌（A-549）癌细胞系的有效抗肿瘤活性，IC _50分别为8.0、7.2分别为4.8和9.1μM。