FNDC4 is an anti-inflammatory factor that alters the activation state of macrophages; it is used to treat colitis in mice. However, its role in muscle formation and mechanism of function remains unknown. We found that FNDC4 promotes the bovine MDSCs migration and differentiation. Furthermore, we reported that it interacts with integrin β1 (ITGβ1). FAK, mediated by ITGβ1, regulates cell migration. Our results found FNDC4 to influence the expression of p-FAK, p-paxillin, and vinculin. Then, overexpressed or added FNDC4 protein could not influence migration and differentiation any more when the activated form of FAK was reduced. Therefore, we concluded that FNDC4 promotes the differentiation and migration of bovine MDSCs via the FAK, mediated by the ITGβ1 receptor.

中文翻译：

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含有纤连蛋白 III 型结构域的 4 通过粘着斑激酶促进牛骨骼肌卫星细胞的迁移和分化。

FNDC4 是一种抗炎因子，可以改变巨噬细胞的活化状态；它用于治疗小鼠结肠炎。然而，它在肌肉形成和功能机制中的作用仍然未知。我们发现 FNDC4 促进牛 MDSCs 迁移和分化。此外，我们报告说它与整合素 β1 (ITGβ1) 相互作用。FAK 由 ITGβ1 介导，调节细胞迁移。我们的结果发现 FNDC4 影响 p-FAK、p-桩蛋白和纽蛋白的表达。然后，当 FAK 的活化形式减少时，过表达或添加的 FNDC4 蛋白不再影响迁移和分化。因此，我们得出结论，FNDC4 通过 ITGβ1 受体介导的 FAK 促进牛 MDSC 的分化和迁移。