AIM To evaluate the biological properties of experimental sealers based on tricalcium silicate and dicalcium silicate, manipulated with polyethylene glycol (CE-1) and with the addition of calcium hypochlorite (CE-2) compared to AH Plus (AHP) and TotalFill BC Sealer (TBC). METHODOLOGY The tissue reaction caused by the materials in the subcutaneous tissue of rats was evaluated after implantation of polyethylene tubes filled with the materials at 7, 15, 30 and 60 days. Sections were stained with haematoxylin and eosin (HE) for morphological analysis and to evaluated number of inflammatory cells/mm2 (ICs). Sections were used for immunohistochemical detection of interleukin-6 (IL-6) and osteocalcin (OC). The von Kossa method was used to identify calcium precipitation in the capsules. The data were submitted to anova and Tukey's tests, with 5% significance level. RESULTS At 7 days, CE-1, CE-2 and AHP had higher numbers of ICs. AHP presented higher immunolabelling for IL-6. After 15 days, regarding IL-6, there was no difference between CE-2 and the control group. At 30 days, AHP exhibited the highest number of IC (P < 0.05) and CE-2 and the control group presented the lowest ICs and IL-6-positive cells. After 60 days, all materials exhibited decreases in ICs. CE-2, TBC and the control had the lowest values (P < 0.05). No significant difference was detected between CE-1 and TBC, and between CE-2 and control considering the immunoexpression of IL-6. In this period, AHP had the greatest number of IC and IL-6 (P < 0.05). In all periods, CE-1, CE-2 and TBC sealers had von Kossa-positive structures and OC-immunolabelled cells. CE-2 had higher number of OC-positive cells than the CE-1 and TBC sealers (P < 0.05), in all periods. OC immunolabelling was not observed in the capsules of AH Plus and the control groups. CONCLUSIONS The experimental sealer and its association with calcium hypochlorite, in addition to TotalFill BC Sealer, were biocompatible and had bioactive potential.

中文翻译：

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评估两种实验性硅酸钙封闭剂的生物学特性：大鼠体内研究。

目的评估与AH Plus（AHP）和TotalFill BC Sealer相比，用聚乙二醇（CE-1）和次氯酸钙（CE-2）处理的基于硅酸三钙和硅酸二钙的实验性密封剂的生物学特性。 TBC）。方法学在植入第7天，第15天，第30天和第60天的聚乙烯管植入填充材料后，评估由大鼠皮下组织中的材料引起的组织反应。将切片用苏木精和曙红（HE）染色以进行形态分析，并评估炎性细胞数/ mm2（ICs）。将切片用于白介素6（IL-6）和骨钙素（OC）的免疫组织化学检测。von Kossa方法用于鉴定胶囊中的钙沉淀。数据已提交给方差分析和Tukey的测试，显着性水平为5％。结果在第7天，CE-1，CE-2和AHP的IC数量更高。AHP提出了更高的IL-6免疫标记。15天后，关于IL-6，CE-2与对照组之间没有差异。在30天时，AHP表现出最高的IC（P <0.05）和CE-2数量，而对照组则表现出最低的IC和IL-6阳性细胞。60天后，所有材料的IC均下降。CE-2，TBC和对照具有最低值（P <0.05）。考虑到IL-6的免疫表达，在CE-1和TBC之间以及在CE-2和对照之间未检测到显着差异。在此期间，AHP的IC和IL-6数量最多（P <0.05）。在所有时期，CE-1，CE-2和TBC封闭剂均具有von Kossa阳性结构和OC免疫细胞。在所有时期中，CE-2的OC阳性细胞数量均高于CE-1和TBC封闭剂（P <0.05）。在AH Plus胶囊和对照组中未观察到OC免疫标记。结论除TotalFill BC密封剂外，该实验密封剂及其与次氯酸钙的结合具有生物相容性，并具有生物活性。