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Rpg7: A New Gene for Stem Rust Resistance from Hordeum vulgare ssp. spontaneum
Phytopathology ( IF 2.6 ) Pub Date : 2021-02-10 , DOI: 10.1094/phyto-08-20-0325-r
Eva Henningsen 1 , Ahmad H Sallam 1 , Oadi Matny 1 , Tamas Szinyei 1 , Melania Figueroa 2 , Brian J Steffenson 1, 3
Affiliation  

Wheat stem rust (causal organism: Puccinia graminis f. sp. tritici) is an important fungal disease that causes significant yield losses in barley. The deployment of resistant cultivars is the most effective means of controlling this disease. Stem rust evaluations of a diverse collection of wild barley (Hordeum vulgare ssp. spontaneum) identified two Jordanian accessions (WBDC094 and WBDC238) with resistance to a virulent pathotype (P. graminis f. sp. tritici HKHJC) from the United States. To elucidate the genetics of stem rust resistance, both accessions were crossed to the susceptible landrace Hiproly. Segregation ratios of F2 and F3 progeny indicated that a single dominant gene confers resistance to P. graminis f. sp. tritici HKHJC. Molecular mapping of the resistance locus was performed in the Hiproly/WBDC238 F2 population based on 3,329 single-nucleotide polymorphism markers generated by genotyping-by-sequencing. Quantitative trait locus analysis positioned the resistance gene to the long arm of chromosome 3H between the physical/genetic positions of 683.8 Mbp/172.9 cM and 693.7 Mbp/176.0 cM. Because this resistance gene is novel, it was assigned the new gene locus symbol of Rpg7 with a corresponding allele symbol of Rpg7.i. At the seedling stage, Rpg7 confers resistance against a number of other important P. graminis f. sp. tritici pathotypes from the United States (MCCFC, QCCJB, and TTTTF) and Africa (TTKSK) as well as an isolate (92-MN-90) of the rye stem rust pathogen (P. graminis f. sp. secalis) from Minnesota. The resistance conferred by Rpg7 can be readily transferred into breeding programs because of its simple inheritance and clear phenotypic expression.



中文翻译:

Rpg7:来自大麦属植物的一种新的抗茎锈病基因。自发性

小麦秆锈病(因果有机体:禾柄锈菌... F SP小麦)是一种重要的真菌疾病,它引起在大麦显著的产量损失。部署抗性品种是控制这种疾病的最有效手段。干野生大麦的不同的收藏品(防锈评价大麦SSP。根子2个约旦种质(WBDC094和WBDC238)有一个致命的致病型电阻标识)(P.禾楼属小麦来自美国HKHJC)。为了阐明抗茎锈病的遗传学,两个种系均与易感的地方品种Hiproly杂交。F 2和F 3的偏析比后代表明单个显性基因赋予了对P. graminis f。的抗性。sp。小麦HKHJC。在Hiproly / WBDC238 F 2群体中,基于通过序列基因分型产生的3,329个单核苷酸多态性标记,对耐药基因座进行了分子定位。数量性状基因座分析将抗性基因定位于68H Mbp / 172.9 cM和693.7 Mbp / 176.0 cM的物理/遗传位置之间对3H染色体长臂的抗性基因。由于该抗性基因是新的,因此为它分配了Rpg7的新基因座符号和相应的Rpg7.i等位基因符号。在苗期,RPG7赋予了对许多其他重要抗性的抵抗力P.格拉米尼斯f。sp。小麦致病型从美国(MCCFC,QCCJB,和TTTTF)和非洲(TTKSK)以及黑麦茎锈病病原体的分离物(92-MN-90)(PF。菌属secalis从明尼苏达州)。Rpg7赋予的抗性由于其简单的遗传和清晰的表型表达,可以很容易地转移到育种程序中。

更新日期:2021-03-19
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