This study sought to evaluate biofilm elimination using the HBW Ultrasonic Ring based on continuous ultrasonic irrigation. Forty-five premolars and molars with complex curvatures were included. An Enterococcus faecalis biofilm was established for 30 days on the extracted teeth. The teeth were then stratified into three experimental groups for instrumentation and irrigation (i.e. HBW Ultrasonic Ring, conventional irrigation, and passive ultrasonic irrigation). Pre- and post-instrumentation samples were collected, and reductions of bacterial load were evaluated by McFarland’s scale, counting of colony-forming units, and scanning electronic microscopy. The HBW Ultrasonic Ring promoted a higher reduction in bacterial load relative to conventional irrigation (P < 0.05) and a similar reduction compared with passive ultrasonic irrigation (P > 0.05). These results suggest the HBW Ultrasonic Ring is a promising alternative modality for simultaneous instrumentation and irrigation during root canal treatment, achieving an appropriate level of bacterial reduction and allowing the passage of the irrigating solution throughout the entire working length.

这项研究试图评估基于连续超声波冲洗的HBW超声波环对生物膜的消除作用。包括45个曲率复杂的前磨牙和磨牙。一个粪肠球菌建立了提取出的牙齿30天生物膜。然后将牙齿分为用于器械和冲洗的三个实验组（即HBW超声环，常规冲洗和被动超声冲洗）。收集仪器前和仪器后的样品，并通过麦克法兰（McFarland's scale），菌落形成单位计数和扫描电子显微镜评估细菌负荷的减少。相对于常规灌溉，HBW超声波环促进了更高的细菌载量减少（P<0.05），与被动超声波灌溉相比，减少幅度相似（P > 0.05）。这些结果表明，HBW超声环是一种有前途的替代方法，可在根管治疗期间同时进行器械和冲洗，实现适当程度的细菌减少，并允许冲洗液在整个工作过程中通过。