Graphene oxide‐based nanomaterials are promising for enzyme immobilization due to the possibilities of functionalizing surface. Polyethylenimine‐grafted graphene oxide was constructed as a novel scaffold for immobilization of formate dehydrogenase. Compared with free formate dehydrogenase and graphene oxide adsorbed formate dehydrogenase, thermostability, storage stability, and reusability of polyethylenimine‐grafted graphene oxide‐formate dehydrogenase were enhanced. Typically, polyethylenimine‐grafted graphene oxide‐formate dehydrogenase remained 47.4% activity after eight times’ repeat reaction. The immobilized capacity of the polyethylenimine‐grafted graphene oxide was 2.4‐folds of that of graphene oxide. Morphological and functional analysis of polyethylenimine‐grafted graphene oxide‐formate dehydrogenase was performed and the assembling mechanism based on multi‐level interactions was studied. Consequently, this practical and facile strategy will likely find applications in biosynthesis, biosensing, and biomedical engineering.

中文翻译：

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甲酸脱氢酶在聚乙烯亚胺接枝氧化石墨烯上的固定化及其动力学和稳定性研究


由于表面功能化的可能性，基于氧化石墨烯的纳米材料在酶固定化方面很有前景。聚乙烯亚胺接枝氧化石墨烯被构建为固定甲酸脱氢酶的新型支架。与游离甲酸脱氢酶和氧化石墨烯吸附甲酸脱氢酶相比，聚乙烯亚胺接枝氧化石墨烯甲酸脱氢酶的热稳定性、储存稳定性和可重复使用性得到增强。通常情况下，聚乙烯亚胺接枝的氧化石墨烯甲酸脱氢酶在八次重复反应后仍保持 47.4% 的活性。聚乙烯亚胺接枝氧化石墨烯的固定容量是氧化石墨烯的2.4倍。对聚乙烯亚胺接枝的氧化石墨烯甲酸脱氢酶进行了形态和功能分析，并研究了基于多级相互作用的组装机制。因此，这种实用且简便的策略可能会在生物合成、生物传感和生物医学工程中得到应用。