The vaccines currently available to control infectious bursal disease (IBD) include live-attenuated and inactivated vaccines, immune-complex vaccines, and vaccines consisting of viral constructs of herpesvirus of turkeys genetically engineered to express VP2 surface protein. To evaluate the impact of vaccines on the chicken immune system, 2 animal trials were performed in specific pathogen-free broiler chickens. In trial 1, birds were either vaccinated when they are one-day old with a dual recombinant herpes virus of turkey construct vaccine, expressing VP2 protein of (IBDV) and F protein of Newcastle disease virus, or an immune-complex IBDV vaccine or birds were not vaccinated. At 14, 28, and 35 D, the bursa of Fabricius was collected for bursa:body weight (B:BW) ratio calculation. In trial 2, birds were vaccinated when they were 1-day old according to the same protocol as trial 1, but at day 14, all groups also received a live infectious bronchitis (IB) vaccine. At 0, 7, 14, 21, and 28 days after IB vaccination, birds were tested by ELISA for IB serology and, soon after the last blood sampling, they were euthanized for collection of Harderian glands, trachea, and spleen and testing by flow cytometry for characterization of mononuclear cells. The immune-complex vaccine groups showed significantly lower B:BW ratio, lower IBV antibody titers, and higher mean percentage of CD8+ T cells in the spleen, trachea, and Harderian glands than those in the other experimental groups. The results of the in vivo trials coupled with a depth analysis of the repertoire of parameters involved in the immune response to IBD and IB vaccinations show one vaccine may influence the immune response of other vaccines included in the vaccination program.

中文翻译：

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在使用不同的传染性法氏囊病疫苗后，在接种了传染性支气管炎疫苗的无特定病原体的肉鸡中免疫学参数的改变。

当前可用于控制传染性法氏囊病（IBD）的疫苗包括减毒活疫苗和灭活疫苗，免疫复合疫苗，以及由经过基因工程改造以表达VP2表面蛋白的火鸡疱疹病毒的病毒构建体组成的疫苗。为了评估疫苗对鸡免疫系统的影响，在无病原体的特殊肉鸡中进行了两项动物试验。在试验1中，给小鸡一天大的时候接种了双重重组土耳其疱疹病毒重组疱疹病毒疫苗，表达了（IBDV）VP2蛋白和新城疫病毒的F蛋白，或者是免疫复合IBDV疫苗没有接种疫苗。在14、28和35 D，收集法氏囊的法氏囊用于法氏囊：体重（B：BW）比率的计算。在审判2中，根据与试验1相同的方案，在1日龄的家禽中接种疫苗，但在第14天，所有组也都接受了活的传染性支气管炎（IB）疫苗。IB疫苗接种后第0、7、14、21和28天，通过ELISA对禽类进行IB血清学检测，最后一次采血后不久，对它们实施安乐死以收集哈代腺，气管和脾脏，并通过血流进行检测细胞计数法鉴定单核细胞。与其他实验组相比，免疫复合疫苗组的脾脏，气管和哈代氏腺中的B：BW比，IBV抗体效价明显降低，CD8 + T细胞平均百分比更高。