Cysteine oxidation is important in cellular redox regulation, signaling, and biocatalysis. To understand the biological relevance of cysteine oxidation, it is desirable to identify the proteins involved, the site of the oxidized cysteine, and the relevant oxidation states. Because the thiol of cysteine can be converted to a wide range of oxidation states, mapping these oxidative modifications is challenging. The dynamic and reversible nature of many cysteine oxidation states compounds the difficulty in such proteomic analyses. In this review, we examine methods to detect cysteine sulfenic acid - a particularly challenging functional group to analyze because of its reactive nature. We focus on the selectivity of recently reported probes and discuss some challenges and opportunities in this field.

中文翻译：

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半胱氨酸次磺酸探针的关键评估。

半胱氨酸氧化在细胞氧化还原调节、信号传导和生物催化中很重要。为了理解半胱氨酸氧化的生物学相关性，需要确定所涉及的蛋白质、氧化半胱氨酸的位点以及相关的氧化态。由于半胱氨酸的硫醇可以转化为多种氧化态，因此绘制这些氧化修饰的图谱具有挑战性。许多半胱氨酸氧化态的动态和可逆性质加剧了此类蛋白质组学分析的困难。在这篇综述中，我们研究了检测半胱氨酸次磺酸的方法 - 由于其反应性质，这是一种特别难以分析的官能团。我们专注于最近报道的探针的选择性，并讨论该领域的一些挑战和机遇。