A single-step copolymerization strategy was developed for the preparation of carbohydrate (glucose and maltose) functionalized monoliths using click reaction. Firstly, novel carbohydrate-functionalized methacrylate monomers were synthesized through Cu(I)-catalyzed 1,3-dipolar cycloaddition (alkyne-azide reaction) of terminal alkyne with azide of carbohydrate derivatives. The corresponding carbohydrate functionalized monolithic columns were then prepared through a single-step in-situ copolymerization. The physicochemical properties and performance of the fabricated monolithic columns were evaluated using scanning electron microscopy, Fourier-transform infrared spectroscopy, and nano-liquid chromatography. For the optimized monolithic column, satisfactory column permeability and good separation performance were demonstrated for polar compounds including nucleoside, phenolic compounds and benzoic acid derivatives. The monolithic column is also highly useful for selective and efficient enrichment of glycopeptides from human IgG tryptic digests. This study not only provided a novel hydrophilic column for separation and selective trapping of polar compounds, but also proposed a facile and efficient approach for preparing carbohydrate functionalized monoliths.

中文翻译：

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一步一步制备碳水化合物官能化整体料，用于分离和捕集极性化合物。

开发了一种单步共聚策略，用于使用点击反应制备碳水化合物（葡萄糖和麦芽糖）官能化的整料。首先，通过末端炔与碳水化合物衍生物的叠氮化物的Cu（I）催化的1,3-偶极环加成反应（炔-叠氮化物反应）合成了新型的碳水化合物官能化的甲基丙烯酸酯单体。然后通过一步原位共聚制备相应的碳水化合物官能化整体柱。使用扫描电子显微镜，傅立叶变换红外光谱和纳米液相色谱法评估了所制备整体柱的理化性质和性能。对于优化的整体柱，极性化合物包括核苷，酚类化合物和苯甲酸衍生物均具有令人满意的色谱柱渗透性和良好的分离性能。整体柱对于从人IgG胰蛋白酶消化物中选择性和有效富集糖肽也非常有用。这项研究不仅为分离和选择性捕集极性化合物提供了一种新型的亲水性色谱柱，而且为制备碳水化合物官能化的整体填料提供了一种简便而有效的方法。