Background: Buyang Huanwu Tang (BYHWT) and relevant Traditional Chinese medicine (TCM) has its unique advantages in the treatment of cerebral ischemia. However, its pharmacological mechanism has not been fully explained.

Objective: Base on the multi-component, also the entire disease network targets, the present study sets out to identify major bioactive ingredients, key disease targets, and pathways of BYHWT against cerebral ischemia disease by systematic pharmacological methodology.

Methods: Both the bioactive compounds from the BYHWT and the positive drugs against cerebral ischemia were fully investigated. The binding targets of the positive drugs were then obtained. A virtual screening protocol was then used to highlight the compound-target interaction and network was constructed to visualize the compound-target binding effect after docking analysis. Moreover, the targets enrichment analysis for biological processes and pathways were performed to further explore the function of bio-targets protein gene and its role in the signal pathway.

中文翻译：

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系统药理方法揭示补阳还五汤治疗脑缺血的作用机制

背景：补阳还五汤（BYHWT）及相关中药（TCM）在治疗脑缺血方面有其独特的优势。但其药理机制尚未完全阐明。

目的：本研究基于多组分、全疾病网络靶点，通过系统药理学方法确定补阳生平抗脑缺血性疾病的主要生物活性成分、关键疾病靶点和途径。

方法：对来自BYHWT的生物活性化合物和抗脑缺血的阳性药物进行了充分的研究。然后获得阳性药物的结合靶标。然后使用虚拟筛选协议来突出化合物-靶标相互作用，并构建网络以在对接分析后可视化化合物-靶标结合效果。此外，对生物过程和通路进行靶标富集分析，以进一步探索生物靶标蛋白基因的功能及其在信号通路中的作用。