Our studies found that melatonin mediates the monochromatic light-induced lymphocyte proliferation in chickens. However, melatonin receptor subtypes contain membrane receptor (Mel1a/Mel1b/Mel1c) and nuclear receptor (Retinoic acid receptor-related orphan receptor [ROR] α/RORβ/RORγ) and are characteristic with cell specificity. This study compared receptor pathway of melatonin, which mediated the monochromatic light-induced T/B lymphocyte proliferations in chickens. Newly hatched chicks were randomly divided into white light, red light, green light (GL), and blue light groups. Green light promoted the membrane receptor expression in the spleen but decreased the nuclear receptor level compared with that of red light. These changes were accompanied by increase of T/B lymphocyte proliferation and plasma melatonin level under GL. Pinealectomy reversed aforementioned changes and resulted in no differences among the light-treated groups. Supplementation of exogenous melatonin enhanced GL-induced T/B lymphocyte proliferation in the spleen but was reversed by Mel1c antagonist prazosin and RORα agonist SR1078 and enhanced by RORα antagonist SR3335. However, Mel1b antagonist 4P-PDOT and RORγ antagonist GSK increased the stimulation effect of melatonin on GL-induced T lymphocyte proliferation but no effect on the B-lymphocyte proliferation. These results indicate that melatonin promotes the GL-induced T lymphocyte proliferation through Mel1b, Mel1c, and RORα/RORγ; however, the Mel1a, Mel1c, and RORα may be involved in the B lymphocyte proliferation.

中文翻译：

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褪黑素通过膜受体或核受体介导脾脏中单色光诱导的T / B淋巴细胞增殖。

我们的研究发现褪黑激素介导了单色光诱导的鸡淋巴细胞增殖。然而，褪黑激素受体亚型包含膜受体（Mel1a / Mel1b / Mel1c）和核受体（视黄酸受体相关的孤儿受体[ROR]α/RORβ/RORγ），具有细胞特异性。这项研究比较了褪黑激素的受体途径，褪黑激素介导了单色光诱导的鸡T / B淋巴细胞增殖。新孵出的小鸡被随机分为白光，红光，绿光（GL）和蓝光组。与红光相比，绿光促进了脾脏膜受体的表达，但降低了核受体的水平。这些变化伴随着GL下T / B淋巴细胞增殖和血浆褪黑激素水平的增加。松果切除术扭转了上述变化，并且在光治疗组之间没有差异。补充外源褪黑激素可增强GL诱导的脾脏T / B淋巴细胞增殖，但被Mel1c拮抗剂prazosin和RORα激动剂SR1078逆转，并被RORα拮抗剂SR3335增强。但是，Mel1b拮抗剂4P-PDOT和RORγ拮抗剂GSK增强了褪黑激素对GL诱导的T淋巴细胞增殖的刺激作用，但对B淋巴细胞增殖没有影响。这些结果表明褪黑激素通过Mel1b，Mel1c和RORα/RORγ促进GL诱导的T淋巴细胞增殖。但是，Mel1a，Mel1c和RORα可能参与了B淋巴细胞的增殖。补充外源褪黑激素可增强GL诱导的脾脏T / B淋巴细胞增殖，但被Mel1c拮抗剂prazosin和RORα激动剂SR1078逆转，并被RORα拮抗剂SR3335增强。但是，Mel1b拮抗剂4P-PDOT和RORγ拮抗剂GSK增强了褪黑激素对GL诱导的T淋巴细胞增殖的刺激作用，但对B淋巴细胞增殖没有影响。这些结果表明褪黑激素通过Mel1b，Mel1c和RORα/RORγ促进GL诱导的T淋巴细胞增殖。但是，Mel1a，Mel1c和RORα可能参与了B淋巴细胞的增殖。补充外源褪黑激素可增强GL诱导的脾脏T / B淋巴细胞增殖，但被Mel1c拮抗剂prazosin和RORα激动剂SR1078逆转，并被RORα拮抗剂SR3335增强。但是，Mel1b拮抗剂4P-PDOT和RORγ拮抗剂GSK增强了褪黑激素对GL诱导的T淋巴细胞增殖的刺激作用，但对B淋巴细胞增殖没有影响。这些结果表明褪黑激素通过Mel1b，Mel1c和RORα/RORγ促进GL诱导的T淋巴细胞增殖。但是，Mel1a，Mel1c和RORα可能参与了B淋巴细胞的增殖。Mel1b拮抗剂4P-PDOT和RORγ拮抗剂GSK增强了褪黑激素对GL诱导的T淋巴细胞增殖的刺激作用，但对B淋巴细胞增殖没有影响。这些结果表明褪黑激素通过Mel1b，Mel1c和RORα/RORγ促进GL诱导的T淋巴细胞增殖。但是，Mel1a，Mel1c和RORα可能参与了B淋巴细胞的增殖。Mel1b拮抗剂4P-PDOT和RORγ拮抗剂GSK增强了褪黑激素对GL诱导的T淋巴细胞增殖的刺激作用，但对B淋巴细胞增殖没有影响。这些结果表明褪黑激素通过Mel1b，Mel1c和RORα/RORγ促进GL诱导的T淋巴细胞增殖。但是，Mel1a，Mel1c和RORα可能参与了B淋巴细胞的增殖。