Subclinical necrotic enteritis (NE) was induced in broiler chicks using a high dose of Eimeria spp. vaccine in the drinking water on day 9, and Clostridium perfringens (Cp) culture mixed in the feed on days 14 and 15. The aim was to evaluate the effects of probiotic Bacillus amyloliquefaciens strain H57 (H57) in preventing NE in chicks. Day-old Ross 308, male broilers were weighed and randomly assigned to 6 treatment groups (6 replicate cages/treatment and 8 birds/cage). Birds in group 1 (control) were fed the basal wheat-soybean diet without H57 or NE infection; in group 2 (Eimeria) were treated with Eimeria alone; in group 3 (Cp) were treated with Cp alone; in group 4 (NE) received both Eimeria and Cp; in group 5 (NE-H57) received NE infection and H57; and group 6 (H57) received H57. The basal diet of chicks in groups 5 and 6 was supplemented with H57 at a density of 2 × 108 spores/g feed from 1 D of age. On day 21, there were no significant treatment effects on BW and feed intake between control and H57 birds. However, on day 21, the feed conversion ratio of NE-H57 birds was significantly improved when compared with NE birds (1.28 vs. 1.36; P < 0.001). Birds challenged with NE had a higher occurrence of pasty vent than birds infected with either Eimeria, Cp, or NE-H57 (41 vs. 27 vs. 29 vs. 19%, respectively; P < 0.001). Intestinal lesion scores of NE birds were also higher than those of Eimeria, Cp, and NE-H57 birds (5.67 vs. 2.56 vs. 2.78 vs. 2.10, respectively; P < 0.001) and correlated with pasty vent (Pearson's r = 0.56; P < 0.001). Microscopic evaluation showed mucosal damage and necrosis in NE birds. In contrast, villi from NE-H57 birds were normal, with no damage or infiltration with Eimeria or Cp. H57 appears to be effective in challenged birds, as it maintained epithelial barrier integrity and improved feed efficiency.

中文翻译：

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益生菌解淀粉芽孢杆菌 H57 通过维持肠粘膜完整性和提高饲料效率来改善肉鸡的亚临床坏死性肠炎。


使用高剂量的艾美耳球虫在肉鸡中诱发亚临床坏死性肠炎 (NE)。第 9 天在饮用水中添加疫苗，第 14 天和第 15 天在饲料中混合产气荚膜梭菌 (Cp) 培养物。目的是评估益生菌解淀粉芽孢杆菌菌株 H57 (H57) 在预防雏鸡 NE 方面的效果。日龄 Ross 308 雄性肉鸡称重并随机分配到 6 个处理组（6 个重复笼/处理和 8 只鸡/笼）。第 1 组（对照）的鸡只饲喂基础小麦-大豆日粮，没有 H57 或 NE 感染；第 2 组（艾美耳球虫）仅用艾美耳球虫治疗；第3组(Cp)仅用Cp治疗；第 4 组（NE）同时接受艾美耳球虫和 Cp；第5组（NE-H57）接受NE感染和H57；第6组（H57）接受H57。第 5 组和第 6 组雏鸡的基础日粮从 1 日龄开始添加 H57，密度为 2 × 108 个孢子/克饲料。第 21 天，对照鸡和 H57 鸡之间的体重和采食量没有显着的治疗效果。然而，在第 21 天，NE-H57 鸡的饲料转化率与 NE 鸡相比显着提高（1.28 vs. 1.36；P < 0.001）。接受 NE 攻击的禽类比感染艾美球虫、Cp 或 NE-H57 的禽类出现糊状排气的发生率更高（分别为 41% vs. 27% vs. 29% vs. 19%；P < 0.001）。 NE 禽类的肠道病变评分也高于艾美耳球虫、Cp 和 NE-H57 禽类（分别为 5.67 vs. 2.56 vs. 2.78 vs. 2.10；P < 0.001），并且与糊状排气相关（Pearson's r = 0.56； P < 0.001）。显微镜评估显示 NE 鸟类的粘膜损伤和坏死。相比之下，NE-H57 禽类的绒毛是正常的，没有受到艾美球虫或 Cp 的损害或渗透。 H57 似乎对受到挑战的鸟类有效，因为它保持了上皮屏障的完整性并提高了饲料效率。