Marek's disease virus (MDV) causes T-cell lymphoma in susceptible chicken and is also related to an imbalance of the lipid metabolism. Adiponectin is a circulatory cytokine secreted from adipose tissue and exerts critical metabolic functions. Although the associations between adiponectin and diseases, including lipid disorder and noncardiac vascular diseases, have been reported, little is known about the relationship between MDV infection and adiponectin. Here, we challenged white Leghorns from Marek's disease (MD)-susceptible and MD-resistant lines with MDV at 7 D of age and then explored the body weight and plasma lipoprotein levels at 21 D after MDV infection. Meanwhile, adiponectin and the expression of its receptors were detected using quantitative real-time PCR and Western blot. The results showed that MDV infection induced body weight loss in all the experimental birds. Meanwhile, the concentrations of total cholesterol and high-density lipoprotein were lower after the infection, although there was no significant difference (P > 0.05). However, the infection did not affect adiponectin circulating levels in plasma. MD-susceptible birds had much lower plasma adiponectin than MD-resistant birds (P < 0.01). In abdominal fat, there was no significant difference in adiponectin mRNA level. Still, we observed a significant decrease in adiponectin protein concentration, as well as adipoR1 and adipoR2, at both mRNA and protein levels in the infected compared with the noninfected MD-susceptible chickens. In the spleen, MDV infection significantly reduced the adiponectin mRNA expression but increased the protein in MD-susceptible chickens, which decreased both adipoR1 mRNA expression and protein levels. Also interestingly, the adipoR1 mRNA expression level was significantly increased in MD-susceptible chickens in the liver after MDV infection. All findings in the present study provided interesting insights into adiponectin metabolism in chickens after MDV infection, which helps to advance the understanding of lipid metabolism in response to herpesvirus infection.

中文翻译：

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脂联素及其受体基因的表达对马来氏病病毒感染的白色来亨鸡的反应。

马立克氏病病毒（MDV）引起易感鸡的T细胞淋巴瘤，并且还与脂质代谢失衡有关。脂联素是从脂肪组织分泌的循环细胞因子，并发挥关键的代谢功能。尽管已经报道了脂联素与疾病（包括脂质疾病和非心血管疾病）之间的关联，但是关于MDV感染与脂联素之间的关系知之甚少。在这里，我们用7岁以下的MDV对来自马立克氏病（MD）易感和MD耐药品系的白色来亨鸡进行了攻击，然后研究了MDV感染后21 D的体重和血浆脂蛋白水平。同时，通过实时定量PCR和Western blot检测脂联素及其受体的表达。结果表明，MDV感染导致所有实验鸟类体重减轻。同时，尽管没有显着差异（P> 0.05），但感染后总胆固醇和高密度脂蛋白的浓度较低。但是，感染并未影响血浆中脂联素的循环水平。MD易感禽类的血浆脂联素比MD耐药性禽类低得多（P <0.01）。在腹部脂肪中，脂联素mRNA水平没有显着差异。尽管如此，我们观察到与未感染MD敏感的鸡相比，在感染的mRNA和蛋白水平上，脂联素蛋白浓度以及adipoR1和adipoR2均显着降低。在脾脏里 MDV感染显着降低了脂联素mRNA的表达，但增加了对MD敏感的鸡的蛋白质，从而降低了adipoR1 mRNA的表达和蛋白质水平。同样有趣的是，MDV感染后，肝脏中易感染MD的鸡的adipoR1 mRNA表达水平显着增加。本研究中的所有发现为MDV感染后鸡的脂联素代谢提供了有趣的见解，这有助于增进对疱疹病毒感染反应中脂质代谢的了解。