Avian embryos and related cell lines have found wide applications in basic and applied sciences. The embryonated egg is a great host for monoclonal antibodies and recombinant proteins. Avian cell lines derived from embryonated eggs have been used for the production of transgenic birds and virus inoculation in vaccine preparation. Hitherto, many efforts have been invested to develop efficient avian stem cell culture. Under the conventional conditions, there are various challenges, such as the type of feeder layers, conditioned medium, serum, and growth factors. Researchers have investigated different conditions to solve these problems. Recent studies have shown that targeted strategies using small molecule inhibitors could be used as alternatives to multi-growth factor delivery approaches. Since small molecule inhibitors were used for mammalian pluripotent stem cells (PSC), several kinds of research have examined the effect of the small molecule on self- -renewal and maintenance of avian PSC. Avian PSC can be derived from early blastodermal cells (stage X), circular primoridial germ cells (PGC; stage HH17), gonadal PGC (stage HH28), and embryonic germ cells (EGC; HH28). Previous studies have shown that the use of small molecule drugs such as PD0325901, SB431542, SC1, IDE1, Z-VAD, Blebbistatin, H-1152, and IDE1 could be an efficient method for the derivation of avian stem cells. This mini-review covers the recent development of avian stem cell culture by small molecules.

鸟类胚胎和相关细胞系在基础和应用科学中得到了广泛的应用。胚蛋是单克隆抗体和重组蛋白的重要宿主。源自含胚蛋的禽类细胞系已用于生产转基因鸟类和疫苗制备中的病毒接种。迄今为止，已经投入了许多努力来开发有效的禽类干细胞培养物。在常规条件下，存在各种挑战，例如饲养层的类型、条件培养基、血清和生长因子。研究人员研究了不同的条件来解决这些问题。最近的研究表明，使用小分子抑制剂的靶向策略可用作多生长因子递送方法的替代方案。由于小分子抑制剂被用于哺乳动物多能干细胞（PSC），多种研究已经检验了小分子对鸟类PSC自我更新和维持的影响。禽 PSC 可来源于早期胚盘细胞（X 期）、圆形原始生殖细胞（PGC；HH17 期）、性腺 PGC（HH28 期）和胚胎生殖细胞（EGC；HH28）。先前的研究表明，使用小分子药物如 PD0325901、SB431542、SC1、IDE1、Z-VAD、Blebbistatin、H-1152 和 IDE1 可能是一种有效的鸟类干细胞衍生方法。这篇简短的评论涵盖了小分子禽类干细胞培养的最新发展。禽 PSC 可来源于早期胚盘细胞（X 期）、圆形原始生殖细胞（PGC；HH17 期）、性腺 PGC（HH28 期）和胚胎生殖细胞（EGC；HH28）。先前的研究表明，使用小分子药物如 PD0325901、SB431542、SC1、IDE1、Z-VAD、Blebbistatin、H-1152 和 IDE1 可能是一种有效的鸟类干细胞衍生方法。这篇简短的评论涵盖了小分子禽类干细胞培养的最新发展。禽 PSC 可来源于早期胚盘细胞（X 期）、圆形原始生殖细胞（PGC；HH17 期）、性腺 PGC（HH28 期）和胚胎生殖细胞（EGC；HH28）。先前的研究表明，使用小分子药物如 PD0325901、SB431542、SC1、IDE1、Z-VAD、Blebbistatin、H-1152 和 IDE1 可能是一种有效的鸟类干细胞衍生方法。这篇简短的评论涵盖了小分子禽类干细胞培养的最新发展。IDE1 可能是一种有效的禽类干细胞衍生方法。这篇简短的评论涵盖了小分子禽类干细胞培养的最新发展。IDE1 可能是一种有效的禽类干细胞衍生方法。这篇简短的评论涵盖了小分子禽类干细胞培养的最新发展。