During a large variety of common pathogens, E. coli, P. aeruginosa, MRSA, MRCNS, V. parahaemolyticus, L. monocytogenes and Salmonella are the leading pathogens responsible for large number of human infections and diseases. In this study, a high flux screening based on nucleic acid isothermal amplification technique has been developed. For the 8 common pathogens, species-specific targets had been selected and analyzed for their unique specificity. After optimization, separate LAMP reaction assays had been bioprocessed and integrated into one systematic detection platform, including 8 strips (PCR tubes) and 96-well plates. Eight standard strains verified for the accuracy. Application of the established high flux screening platform was used for detection for 48 samples in 4 different 96-well plates, with 2 groups of 2 operators using double-blind procedure. The accuracy of 100% was obtained, with the total time consumption as 66–75 min (for 12 samples detection on 8 different pathogens). As concluded, through the bioprocess of the systematic platform based on LAMP technique, it’s been demonstrated to be capable of simultaneous detection of 8 pathogens, with high sensitivity, specificity, rapidity and convenience.

在多种常见病原体中，大肠杆菌、铜绿假单胞菌、MRSA、MRCNS、副溶血性弧菌、单核细胞增生李斯特菌和沙门氏菌是造成大量人类感染和疾病的主要病原体。在本研究中，开发了基于核酸等温扩增技术的高通量筛选。对于 8 种常见病原体，选择了物种特异性目标并对其独特的特异性进行了分析。经过优化后，单独的 LAMP 反应测定已被生物处理并集成到一个系统检测平台中，包括 8 个试纸条（PCR 管）和 96 孔板。验证了八个标准菌株的准确性。应用已建立的高通量筛选平台，对4个不同96孔板中的48个样品进行检测，采用双盲程序，分为2组2名操作人员。准确率100%，总耗时66-75分钟（12个样本检测8种不同病原体）。结论表明，通过基于LAMP技术的系统平台的生物处理，可同时检测8种病原体，具有高灵敏度、特异度、快速性和便利性。