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Physiological and metabolic responses in juvenile Colossoma macropomum exposed to hypoxia.
Fish Physiology and Biochemistry ( IF 2.5 ) Pub Date : 2020-08-29 , DOI: 10.1007/s10695-020-00868-8
Luanna do Carmo Neves 1 , Gisele Cristina Favero 1 , Suzane Lilian Beier 2 , Nathália Soares Ferreira 1 , Glauber David Almeida Palheta 3 , Nuno Filipe Alves Correia de Melo 3 , Ronald Kennedy Luz 1, 4
Affiliation  

This study aimed to evaluate hematological, biochemical, and gasometric parameters of tambaqui juveniles (Colossoma macropomum) exposed to hypoxia and subsequent recovery. Six animals were subjected to normoxia (basal) treatment with dissolved oxygen (DO) 6.27 ± 0.42 mg L−1. Water flow and aeration were reduced for 3 days (hypoxia), during which DO was 0.92 ± 0.37 mg L−1. Water flow and aeration were then reestablished with DO remaining similar to basal. The treatments were as follows: normoxia (basal); 24 h after initiating hypoxia (24H); 72 h after initiating hypoxia (72H); 24 h after reestablishing normoxia (24R); 48 h after reestablishing normoxia (48R); and 96 after reestablishing normoxia (96R). The highest glucose level was recorded at 24H (P < 0.05); the highest lactate level was at 72R; and the highest blood pH was at 24H and 72H (P < 0.05). The highest concentration of PvCO2 was at 24H (P < 0.05), while at 96R it was equivalent to basal (P > 0.05). The variable PvO2 was only higher than basal at 24R (P < 0.05). Juvenile C. macropomum managed to reestablish the main stress indicators (glucose and lactate) at 96R, while the other indicators varied during the study, with homeostatic physiology being reestablished during the recovery period.



中文翻译:

暴露于低氧状态下的少年巨像的生理和代谢反应。

这项研究的目的是评估坦巴基少年(血液学,生化和气体容量参数淡水白macropomum缺氧和随后的恢复)。六只动物用6.27±0.42 mg L -1的溶氧(DO)进行常氧(基础)治疗。减少水流量和通气3天(缺氧),在此期间DO为0.92±0.37 mg L -1。然后重新建立水流量和通气状态,DO保持与基础相似。治疗方法如下:常氧(基础);开始缺氧后24小时(24小时);开始缺氧后72小时(72H);恢复常氧(24R)后24小时;恢复常氧(48R)后48小时;恢复正常氧水平(96R)后为96。在24H(P  <0.05); 最高乳酸水平为72R;血液的最高pH在24H和72H(P  <0.05)。PvCO 2的最高浓度在24H(P  <0.05),而在96R时则等于基础浓度(P  > 0.05)。变量PvO 2仅在24R时高于基础值(P  <0.05)。少年梭状芽胞杆菌设法在96R处建立了主要的应激指标(葡萄糖和乳酸),而其他指标在研究期间有所变化,在恢复期恢复了体内稳态生理。

更新日期:2020-10-26
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