The nitrogen-fixing bacterial strain UFLA 01-1174 T was isolated from nodules of Campsiandra laurilifolia Benth. originating from the Amazon region, Brazil. Its taxonomic position was defined using a polyphasic approach. Analysis of the 16S rRNA gene placed the strain in the Bradyrhizobium genus, the closest species being B. guangdongense CCBAU 51649 T and B. guangzhouense CCBAU 51670 T , both with 99.8% similarity. Multilocus sequence analysis (MLSA) of recA , gyrB , glnII , rpoB , atpD , and dnaK indicated that UFLA 01-1174 T is a new species, most closely related to B. stylosanthis BR 446 T (94.4%) and B. manausense BR 3351 T (93.7%). Average nucleotide identity (ANI) differentiated UFLA 01-1174 T from the closest species with values lower than 90%. The G + C content in the DNA of UFLA 01-1174 T is 63.6 mol%. Based on this data, we conclude that the strain represents a new species. The name proposed is Bradyrhizobium campsiandrae , with UFLA 01-1174 T (= INPA 394B T = LMG 10099 T ) as type strain.

中文翻译：

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Bradyrhizobium campsiandrae sp. 11 月，一种从亚马逊原生豆科树中分离出来的固氮细菌菌株，适应了水淹条件

从 Campsiandra laurilifolia Benth 的根瘤中分离出固氮细菌菌株 UFLA 01-1174 T。原产于巴西亚马逊地区。它的分类位置是使用多相方法定义的。16S rRNA 基因分析将该菌株置于慢生根瘤菌属中，最接近的物种是B. guangdongense CCBAU 51649 T 和B. guangzhouense CCBAU 51670 T，两者的相似度为99.8%。recA、gyrB、glnII、rpoB、atpD 和 dnaK 的多位点序列分析 (MLSA) 表明 UFLA 01-1174 T 是一个新物种，与 B. stylosanthis BR 446 T (94.4%) 和 B. manausense BR 关系最密切3351吨（93.7%）。平均核苷酸同一性 (ANI) 将 UFLA 01-1174 T 与最接近的物种区分开来，其值低于 90%。UFLA 01-1174 T 的 DNA 中 G + C 含量为 63.6 mol%。根据这些数据，我们得出结论，该菌株代表了一个新物种。提议的名称是 Bradyrhizobium campsiandrae，UFLA 01-1174 T (= INPA 394B T = LMG 10099 T) 作为类型菌株。