The objective of this study was to compare the effects of nanoselenium (NS) and selenium yeast (SY) on the performance, egg selenium (Se) concentration, and anti-oxidative capacity of hens. A total of 216 Brown Hy-line hens (29-week old) were randomly allocated into three treatments (6 replicate/treatment, 12 hens/replicate). The pre-trial period lasted 7 days, and the experimental period lasted 35 days. Dietary treatments included corn-soybean meal basal diet (containing 0.16 μg Se/g, as control group), and basal diet supplemented with 0.3 mg Se/kg diet (Se was from NS or SY), called as SY group or NS group, respectively. At the end of the experiment, one hen per replicate from each treatment was slaughtered. Liver, spleen, and kidney tissues were sampled for the determination of Se concentrations. The results showed that NS or SY supplement significantly improved feed conversion ratio (P < 0.05), soft broken egg rate (P < 0.05), and the serum T-AOC value (P < 0.05) when compared with control group. Remarkably, the deposition of Se increased significantly (P < 0.05) and equivalently in egg, liver, and kidney of hens supplemented with both NS and SY. Interestingly, SY supplement also enhanced the serum CAT and SOD activities (P < 0.05), NS but not SY significantly reduced serum MDA (P < 0.05), whereas RT-PCR results did not show significant differences in the mRNA levels of antioxidant genes among three groups (P > 0.05). Taken together, dietary supplemented with SY or NS improved the Se deposition in eggs, liver and kidney of laying hens, increased antioxidant activity, and NS supplement had greater Se deposition in the kidney tissue than SY supplement. SY or NS supplement could be considered to be applied for Se-enriched egg production.

中文翻译：

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纳米硒和硒酵母对蛋鸡产蛋量和硒沉积的差异很小。

本研究的目的是比较纳米硒 (NS) 和硒酵母 (SY) 对母鸡的生产性能、鸡蛋硒 (Se) 浓度和抗氧化能力的影响。总共 216 只 Brown Hy-line 母鸡（29 周龄）被随机分配到三个处理中（6 个重复/处理，12 只母鸡/重复）。预审期为7天，实验期为35天。日粮处理包括玉米-豆粕基础日粮（含0.16 μg Se/g，为对照组）和基础日粮补充0.3 mg Se/kg日粮（Se来自NS或SY），称为SY组或NS组，分别。在实验结束时，每个处理的每个重复中屠宰一只母鸡。对肝脏、脾脏和肾脏组织取样以测定硒浓度。结果表明，与对照组相比，添加NS或SY显着提高饲料转化率（P < 0.05）、软破蛋率（P < 0.05）和血清T-AOC值（P < 0.05）。值得注意的是，同时添加 NS 和 SY 的母鸡的鸡蛋、肝脏和肾脏中 Se 的沉积显着增加（P < 0.05）并且相当。有趣的是，SY 补充剂还增强了血清 CAT 和 SOD 活性（P < 0.05），NS 但不是 SY 显着降低了血清 MDA（P < 0.05），而 RT-PCR 结果未显示抗氧化基因的 mRNA 水平存在显着差异。三组（P > 0.05）。综上所述，日粮中添加SY或NS可改善蛋鸡、肝脏和肾脏中硒的沉积，增加抗氧化活性，NS 补充剂在肾脏组织中的 Se 沉积量高于 SY 补充剂。SY或NS补充剂可考虑应用于富硒鸡蛋生产。