Apoptosis is programmed cell death and its alteration is related to cancer, neurologic, autoimmune, and chronic diseases. A number of factors can affect this process. The aim of this paper is to study the effect of supplemental zinc on apoptosis-related genes in C2C12 myoblast cells after being challenged with a series of stimuli, such as high glucose, insulin, and an inflammatory agent. C2C12 myoblast cells were cultured for 24 h with zinc (Zn) (ZnSO₄) 10 or 100 μM and/or glucose 10 or 30 mM. In addition to these stimuli, the cells were challenged with insulin 1 nM or interleukin-6 (IL-6) 5 nM. The mRNA expression of proapoptotic genes caspase 3 and Fas, the antiapoptotic genes, Xiap and Bcl-xL and the ratio of pro-/antiapoptotic genes Bax/Bcl-2, were determined by qRT-PCR. The expression of caspase-3 gene was significantly increased in the presence of the combination high Zn/high glucose with and without the presence of insulin and IL6 in the culture medium Fas expression instead, showed uneven responses. The expression of Bcl-xL and Xiap was increased in most conditions by having high Zn in the medium regardless of the presence of insulin or IL6. Bax/Bcl2 ratio was decreased in the presence of high Zn. Zn was able to stimulate the expression of antiapoptotic genes. This effect was specially noted in high-glucose conditions with and without the presence of insulin. This effect is partially overridden by the presence of an inflammatory agent such as IL-6.

凋亡是程序性细胞死亡，其改变与癌症，神经系统疾病，自身免疫性疾病和慢性疾病有关。许多因素都会影响此过程。本文的目的是研究补充锌对高糖，胰岛素和炎性因子等一系列刺激后对C2C12成肌细胞凋亡相关基因的影响。C2C12成肌细胞与锌（Zn）（ZnSO ₄）10或100μM和/或葡萄糖10或30 mM。除这些刺激外，还用1 nM胰岛素或5 nM白介素6（IL-6）攻击细胞。通过qRT-PCR测定促凋亡基因caspase 3和Fas的mRNA表达，抗凋亡基因Xiap和Bcl-xL以及促/抗凋亡基因Bax / Bcl-2的比例。在培养基中Fas表达的情况下，在有和没有胰岛素和IL6存在的情况下，在高锌/高葡萄糖联合存在下，caspase-3基因的表达显着增加。在大多数条件下，无论是否存在胰岛素或IL6，培养基中的锌含量高，Bcl-xL和Xiap的表达都会增加。在高锌存在下，Bax / Bcl2比降低。锌能够刺激抗凋亡基因的表达。在存在和不存在胰岛素的高葡萄糖条件下，这种效果特别明显。炎症剂（例如IL-6）的存在可部分抵消这种作用。