Introduction. Enterococcus faecalis is a facultative, anaerobic, opportunistic pathogen associated with medical and dental diseases. Bacterial phenotypic traits and pathogenesis are often influenced by lysogeny. Aim. The aim of this study was to characterize both the morphology and complete genome sequences of induced prophages purified from E. faecalis clinical isolates. Methodology. E. faecalis isolates were recovered from the roots of teeth of patients attending an endodontic clinic. The morphological features of isolated phage were characterized using transmission electron microscopy (TEM). DNA sequencing was performed using the Illumina MiSeq platform. Results. TEM indicated that the isolated φEf-vB1 prophage belongs to the family Siphoviridae. The φEf-vB1 prophage was stable over a wide range of temperatures and pH. Sequencing of φEf-vB1 DNA revealed that the phage genome is 37 561 bp in length with a G+C content of 37.6mol% and contained 53 ORFs. Comparison with previously predicted prophage genomes using blast revealed that φEf-vB1 has a high sequence similarity to previously characterized phage genomes. The lysogenic E. faecalis strain exhibited a higher biofilm formation capacity relative to the non-lysogenic strain. Conclusion. The current findings highlight the role of lysogeny in modification of E. faecalis properties and reveal the potential importance of prophages in E. faecalis biology and pathogenesis.

中文翻译：

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表征φEf-vB1噬菌体感染口腔粪肠球菌并增强细菌生物膜形成。

介绍。 粪肠球菌是与医学和牙齿疾病相关的兼性，厌氧，机会病原体。细菌的表型性状和发病机理通常受溶原性影响。目标。这项研究的目的是表征从粪肠球菌临床分离株纯化的诱导原菌的形态和完整的基因组序列。方法。从参加牙髓诊所的患者的牙齿的根中回收了粪肠球菌。分离的噬菌体的形态特征用透射电子显微镜（TEM）表征。使用Illumina MiSeq平台进行DNA测序。结果。 TEM表明，孤立φEf-VB1噬菌体属于家庭长尾噬菌体科。φEf-vB1噬菌体在很宽的温度和pH范围内都是稳定的。φEf-vB1DNA的测序表明，噬菌体基因组的长度为37561 bp，G + C含量为37.6mol％，包含53个ORF。使用blast与先前预测的噬菌体基因组进行比较表明，φEf-vB1与先前表征的噬菌体基因组具有高度的序列相似性。相对于非溶原性菌株，溶原性粪肠球菌菌株显示出更高的生物膜形成能力。结论。目前的研究结果突出了溶菌原在粪肠球菌的修饰中的作用 的特性，并揭示了前噬菌在粪肠球菌生物学和发病机理中的潜在重要性。