Plant-breeding technologies have expanded, accelerating breeding research beyond the confines of current regulations. The application of genome editing, such as CRISPR-Cas9, do not neatly fit into existing regulatory frameworks, creating uncertainty as to whether they can be regarded as conventionally developed varieties without further regulation. This research presents the current views of Canadian plant breeders based on a national survey of plant breeders. There is evidence that a review of existing regulations is required, as >60% anticipate the use of genome-editing technologies in the next few years. This paper reviews plant-breeding practices under the context of present plants with novel trait (PNT) regulations and where plant breeders place the use of CRISPR-Cas9 within the suite of available genome-editing options. This paper establishes when and why, or why not, breeders choose to introduce CRISPR-Cas9 into their research over other plant-breeding applications.

中文翻译：

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CRISPR-Cas9在加拿大公共和私人植物育种中的应用。

植物育种技术已经扩展，加速了育种研究，超出了当前法规的范围。基因组编辑的应用，例如CRISPR-Cas9，不能很好地适应现有的监管框架，从而不确定是否可以将它们视为常规开发的品种而无需进一步的监管。这项研究基于对植物育种者的全国调查，提出了加拿大植物育种者的当前观点。有证据表明，有必要对现有法规进行审查，因为超过60％的专家预计在未来几年内将使用基因组编辑技术。本文在具有新颖性状（PNT）法规的当前植物以及植物育种者将CRISPR-Cas9纳入可用基因组编辑选项套件的情况下回顾了植物育种实践。