The increase in production of cannabis for medical and recreational purposes in recent years has led to a corresponding increase in laboratories performing cannabinoid analysis of cannabis and hemp. We have developed and validated a quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) method that is simple, reliable, specific, and accurate for the analysis of 17 cannabinoids in cannabis and hemp. Liquid-solid sample extraction coupled with dilution into a calibration range from 10 to 10,000 ng/mL and LC-MS/MS analysis provides quantification of samples ranging from 0.002 to 200 mg/g (0.0002 to 20.0%) in matrix. Linearity of calibration curves in methanol was demonstrated with regression r² ≥ 0.99. Within-batch precision (0.5 to 6.5%) and accuracy (91.4 to 108.0%) and between-batch precision (0.9 to 5.1%) and accuracy (91.5 to 107.5%) were demonstrated for quality control (QC) samples in methanol. Within-batch precision (0.2 to 3.6%) and accuracy (85.4 to 111.6%) and between-batch precision (1.4 to 6.1 %) and accuracy (90.2 to 110.3%) were also evaluated with a candidate cannabis certified reference material (CRM). Repeatability (1.5 to 12.4% RSD) and intermediate precision (2.2 to 12.8% RSD) were demonstrated via analysis of seven cannabis samples with HorRat values ranging from 0.3 to 3.1. The method provides enhanced detection limits coupled with a large quantitative range for 17 cannabinoids in plant material. It is suitable for a wide range of applications including routine analysis for delta-9-tetrahydrocannabinol (Δ⁹-THC), delta-9-tetrahydrocannabinolic acid (Δ⁹-THCA), cannabidiol (CBD), cannabidiolic acid (CBDA), and cannabinol (CBN) as well as more advanced interrogation of samples for both major and minor cannabinoids.

近年来，用于医疗和休闲用途的大麻产量增加，导致进行大麻和大麻大麻素分析的实验室相应增加。我们已经开发并验证了定量，液相色谱-串联质谱（LC-MS / MS）方法，该方法简单，可靠，专一且准确，可用于分析大麻和大麻中的17种大麻素。液固样品提取以及稀释至10至10,000 ng / mL的校准范围以及LC-MS / MS分析可对基质中0.002至200 mg / g（0.0002至20.0％）的样品进行定量。回归r ²证明了甲醇中校准曲线的线性 ≥0.99。甲醇中的质量控制（QC）样品的批内精密度（0.5至6.5％）和准确度（91.4至108.0％）和批间精密度（0.9至5.1％）和准确度（91.5至107.5％）被证明。还使用候选大麻认证参考材料（CRM）评估了批内精密度（0.2至3.6％）和准确度（85.4至111.6％）和批间精密度（1.4至6.1％）和准确度（90.2至110.3％） 。通过分析HorRat值在0.3到3.1之间的七个大麻样品，证明了重复性（1.5到12.4％RSD）和中等精度（2.2到12.8％RSD）。该方法提高了植物材料中17种大麻素的检测限，并具有较大的定量范围。它适用于各种应用，包括对delta-9-四氢大麻酚（Δ⁹ -THC），δ-9- tetrahydrocannabinolic酸（Δ ⁹ -THCA），大麻二酚（CBD），大麻二乙酸（CBDA）和大麻酚（CBN）作为主要和次要的大麻素的样品以及更先进的询问。