Industrial production of 2-keto-L-gulonic acid (2-KLG), the precursor of vitamin C, is mainly achieved by a two-step fermentation process carried out by Gluconobacter oxydans, Bacillus, and Ketogulonicigenium. One of the most promising innovations that could replace this complicated two-step fermentation process is the integration of the essential genes for synthesis of 2-KLG into G. oxydans and use of it as the producer. Therefore, determining the tolerance and response of G. oxydans to 2-KLG is a priority for improving the direct production of 2-KLG in this bacterium. In this study, a global view of the gene expression of G. oxydans WSH-003 in response to 2-KLG challenge was investigated by RNA sequencing. A total of 363 genes of G. oxydans that were differentially expressed in response to 2-KLG were uncovered. The results showed that 2-KLG could lead to oxidative stress, osmotic stress, and DNA damage in G. oxydans.

中文翻译：

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暴露于升高的2-酮基-L-古洛糖酸中的氧化葡糖杆菌WSH-003的转录组分析揭示了对渗透压和氧化应激的响应。

维生素C的前体2-酮基-L-古洛糖酸（2-KLG）的工业生产主要是通过氧化葡糖杆菌，芽孢杆菌和酮古隆菌属细菌进行的两步发酵过程实现的。可以取代这种复杂的两步发酵过程的最有前途的创新之一是将2-KLG合成所必需的基因整合到氧化单胞菌中，并将其用作生产者。因此，确定氧化羟色胺对2-KLG的耐受性和应答是改善2-KLG在该细菌中直接产生的优先事项。在这项研究中，通过RNA测序研究了氧化羟色胺WSH-003基因响应2-KLG攻击的基因表达的整体观点。总共发现了对2-KLG响应差异表达的oxy。G. oxydans基因363。