Neural guidance mechanisms ensure the precise targeting and synaptogenesis events essential for normal circuit function. Neuronal growth cones encounter numerous attractive and repulsive cues as they navigate toward their intermediate and final targets; temporal and spatial regulation of these responses are critical for circuit assembly. Recent work highlights the complexity of these events throughout neural development and the multifaceted functions of a wide range of guidance cues. Here, we discuss recent studies that leverage advances in genetics, single cell tracing, transcriptomics and proteomics to further our understanding of the molecular mechanisms underlying neural guidance and overall circuit organization.

中文翻译：

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重新审视和完善神经引导线索在电路组装中的作用。


神经引导机制确保正常电路功能所必需的精确定位和突触发生事件。神经元生长锥在朝着中间和最终目标前进时会遇到许多吸引和排斥的线索；这些响应的时间和空间调节对于电路组装至关重要。最近的工作强调了这些事件在整个神经发育过程中的复杂性以及各种指导线索的多方面功能。在这里，我们讨论最近的研究，这些研究利用遗传学、单细胞追踪、转录组学和蛋白质组学的进展来进一步了解神经引导和整体回路组织的分子机制。