Abstract

Soil adulteration by organic and inorganic contaminants chiefly in industrial and agricultural area is one of the major problems faced by the world today. Phytoremediation using aromatic plant such as Ocimum is a sound, economically reasonable and an eco-friendly approach. Besides, microbial assisted phytoremediation can bring about plant-promoted microbial degradation, plant uptake and phytodegradation of soil contaminants. In this study, the ability of Ocimum gratissimum in association with Pseudomonas putida MU02 to remediate zinc and crude oil contaminated soil (2,000 mg/kg, 1% w w⁻¹ crude oil) was investigated in a 60-day pot experiment. Four different treatments and a control (clean soil + plant) were employed for the study: co-contaminated soil only; co-contaminated soil with O. gratissimum; co-contaminated with P. putida inoculum; co-contaminated soil with O. gratissimum and P. putida. Comparatively, the highest TPH deduction efficiency (75.87%) was shown by the treatment comprising of O. gratissimum and P. putida while highest metal uptake was shown by the treatment with plant only. Better TPH removal associated with higher number of bacteria in the vegetated and inoculated pots could be attributed to the rhizopheric effect of the plants. Altogether, the association of O. gratissimum with P. putida can be a potential candidate for the remediation of co-contaminated soil.

摘要

主要由工业和农业地区的有机和无机污染物引起的土壤掺假是当今世界面临的主要问题之一。使用芳香植物（如Ocimum）进行植物修复是一种合理，经济合理且环保的方法。此外，微生物辅助的植物修复可以引起植物促进的微生物降解，植物吸收和土壤污染物的植物降解。在这项研究中，Ocimum gratissimum与恶臭假单胞菌MU02联合修复锌和原油污染土壤的能力（2,000 mg / kg，1％ww ^-1原油）在60天的锅内实验中进行了研究。四种不同的处理方法和对照（清洁土壤+植物）用于研究：仅被共同污染的土壤；与O. gratissimum共污染的土壤；与恶臭假单胞菌接种物共同污染；被O. gratissimum和P. putida共同污染的土壤。相比之下，由O. gratissimum和P. putida组成的处理显示出最高的TPH扣除效率（75.87％）而仅用植物处理显示出最高的金属吸收。在植物盆栽和接种盆中，更好的TPH去除与更多细菌相关联可以归因于植物的发根效果。总的来说，O。gratissimum与恶臭假单胞菌的关联可能是修复受污染土壤的潜在候选者。