In general, a sufficient supply of ATP can promote the synthesis of ATP-driven metabolites, but excessive ATP will lead to the inhibition of cell growth. For enhancing the co-production of glutathione(GSH) and S-adenosylmethionine(SAM), a dynamic ATP regeneration strategy was developed. The novel ATP regeneration strategy consisting of ATP-sensing riboswitch ydaO motif, polyphosphate kinase (PPK), and Vitreoscilla hemoglobin (VHb) was successfully applied in Escherichia coli. The intracellular ATP level was always around 0.60 mg/g dry cell weight during the fermentation process, resulting in significantly enhanced co-production of GSH and SAM. The GSH titer and SAM titer in the strain CGS-2 increased by 137.40% and 82.18% after fermentation for 24 h, compared with the control strain. The ATP regulation strategy is expected to be a favorable tool to improve the efficiency of microbial cell factories. The proposed ATP dynamic regeneration approach may be applicable for cost-effective, high-yield production of ATP-driven metabolites.

中文翻译：

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提高大肠杆菌中谷胱甘肽和S-腺苷甲硫氨酸联合生产的ATP动态再生策略

一般来说，充足的 ATP 供应可以促进 ATP 驱动代谢物的合成，但过多的 ATP 会导致细胞生长受到抑制。为了提高谷胱甘肽 (GSH) 和 S-腺苷甲硫氨酸 (SAM) 的协同生产，开发了一种动态 ATP 再生策略。由 ATP 传感核糖开关 ydaO 基序、多磷酸激酶 (PPK) 和玻璃体血红蛋白 (VHb) 组成的新型 ATP 再生策略已成功应用于大肠杆菌。在发酵过程中，细胞内 ATP 水平始终在 0.60 mg/g 细胞干重左右，导致 GSH 和 SAM 的联合生产显着增强。与对照菌株相比，发酵24 h后菌株CGS-2的GSH效价和SAM效价分别提高了137.40%和82.18%。ATP 调控策略有望成为提高微生物细胞工厂效率的有利工具。提议的 ATP 动态再生方法可能适用于 ATP 驱动的代谢物的成本效益高、产量高的生产。