In this contribution we present a new algorithm for structured illumination microscopy with incoherent light. Existing algorithms for determining the contrast values of the focal depth response require a high accurate phase shift of the fringe pattern illumination. The presented algorithm, which is robust against inaccurate phase shift of the fringe pattern, reduces significantly the requirements for the phase shift and consequently the costs of the microscope. The new algorithm was tested by a preliminary experiment, whereby the grating was shifted by an elastic guided micro-motion mechanism employing a low-cost stepper motor replacing the conventional expensive piezo drive. The determined focal depth response is very smooth and corresponds very well to the theoretical focal depth response.

在这一贡献中，我们提出了一种用于非相干光的结构化照明显微镜的新算法。用于确定焦深响应的对比度值的现有算法需要条纹图案照明的高精度相移。所提出的算法对条纹图案的不精确相移具有鲁棒性，该算法显着降低了相移的要求，并因此降低了显微镜的成本。该新算法通过初步实验进行了测试，其中光栅通过弹性导向微动机构移动，该机构采用低成本的步进电机代替了传统的昂贵压电驱动器。所确定的焦深响应非常平滑，并且与理论焦深响应非常吻合。