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Green synthesis of highly luminescent biotin-conjugated CdSe quantum dots for bioimaging applications
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2020-09-10 , DOI: 10.1039/d0nj03075a Vijaya Bharathi M. 1, 2, 3, 4, 5 , Nilmadhab Roy 3, 4, 5, 6, 7 , Prithvi Moharana 3, 4, 5, 6, 7 , Kaustab Ghosh 1, 2, 3 , Priyankar Paira 3, 4, 5, 6, 7
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2020-09-10 , DOI: 10.1039/d0nj03075a Vijaya Bharathi M. 1, 2, 3, 4, 5 , Nilmadhab Roy 3, 4, 5, 6, 7 , Prithvi Moharana 3, 4, 5, 6, 7 , Kaustab Ghosh 1, 2, 3 , Priyankar Paira 3, 4, 5, 6, 7
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Semiconductor nanoparticles with very small sizes of 2–20 nm (quantum dots) are very much attractive for their excellent photoluminescence property. Herein, this study portrays the green synthesis of highly fluorescent, water-soluble, semiconducting CdSe quantum dots (CdSe QDs) as the most potential cellular imaging tag. The stability of CdSe quantum dots has been achieved by capping with mercaptopropionic acid (MPA) as a stabilising agent, and then biotin was attached to quantum dots after surface modification with streptravidin to attain selective cancer cell targeting ability with enhanced conjugation efficiency, which can result in the easy detection of cancer cells growing in our body. The formation of the desired biotin-conjugated highly luminescent CdSe quantum dot has been well justified by photoluminescence (PL), ultraviolet-visible (UV-vis) and Fourier transform infrared (FTIR), X-ray diffraction (XRD), and transmission electron microscopy (TEM) techniques. The cancer cell imaging quality of the biotin-conjugated CdSe QD has been assessed with respect to the Human Epitheloid Cervix Carcinoma (HeLa) cell. Cytotoxicity and cellular imaging results in both cancerous HeLa and normal HEK-293 cells revealed the efficiency of this material as an imaging probe.
中文翻译：
用于生物成像应用的高发光生物素共轭CdSe量子点的绿色合成
具有2-20 nm（量子点）非常小的尺寸的半导体纳米粒子因其出色的光致发光特性而非常吸引人。在本文中，这项研究描绘了绿色荧光合成的高荧光，水溶性，半导体CdSe量子点（CdSe QDs）作为最有潜力的细胞成像标签。CdSe量子点的稳定性通过巯基丙酸（MPA）作为稳定剂的封端来实现，然后在用链霉亲和素进行表面修饰后将生物素附着到量子点上，从而获得具有增强结合效率的选择性癌细胞靶向能力，这可能导致易于检测我们体内生长的癌细胞。所需的生物素共轭的高发光CdSe量子点的形成已通过光致发光（PL）得到充分证明，紫外可见（UV-vis）和傅立叶变换红外（FTIR），X射线衍射（XRD）和透射电子显微镜（TEM）技术。已针对人上皮子宫颈癌（HeLa）细胞评估了生物素共轭CdSe QD的癌细胞成像质量。在癌性HeLa和正常HEK-293细胞中的细胞毒性和细胞成像结果表明该材料作为成像探针的效率。
更新日期：2020-10-15
中文翻译：
用于生物成像应用的高发光生物素共轭CdSe量子点的绿色合成
具有2-20 nm（量子点）非常小的尺寸的半导体纳米粒子因其出色的光致发光特性而非常吸引人。在本文中，这项研究描绘了绿色荧光合成的高荧光，水溶性，半导体CdSe量子点（CdSe QDs）作为最有潜力的细胞成像标签。CdSe量子点的稳定性通过巯基丙酸（MPA）作为稳定剂的封端来实现，然后在用链霉亲和素进行表面修饰后将生物素附着到量子点上，从而获得具有增强结合效率的选择性癌细胞靶向能力，这可能导致易于检测我们体内生长的癌细胞。所需的生物素共轭的高发光CdSe量子点的形成已通过光致发光（PL）得到充分证明，紫外可见（UV-vis）和傅立叶变换红外（FTIR），X射线衍射（XRD）和透射电子显微镜（TEM）技术。已针对人上皮子宫颈癌（HeLa）细胞评估了生物素共轭CdSe QD的癌细胞成像质量。在癌性HeLa和正常HEK-293细胞中的细胞毒性和细胞成像结果表明该材料作为成像探针的效率。
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