GetOrganelle is a state-of-the-art toolkit to accurately assemble organelle genomes from whole genome sequencing data. It recruits organelle-associated reads using a modified “baiting and iterative mapping” approach, conducts de novo assembly, filters and disentangles the assembly graph, and produces all possible configurations of circular organelle genomes. For 50 published plant datasets, we are able to reassemble the circular plastomes from 47 datasets using GetOrganelle. GetOrganelle assemblies are more accurate than published and/or NOVOPlasty-reassembled plastomes as assessed by mapping. We also assemble complete mitochondrial genomes using GetOrganelle. GetOrganelle is freely released under a GPL-3 license ( https://github.com/Kinggerm/GetOrganelle ).

中文翻译：

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GetOrganelle：快速、多功能的工具包，用于准确地从头组装细胞器基因组


GetOrganelle 是一款最先进的工具包，可根据全基因组测序数据准确组装细胞器基因组。它使用修改后的“诱饵和迭代映射”方法招募细胞器相关的读数，进行从头组装、过滤和解开组装图，并产生圆形细胞器基因组的所有可能配置。对于 50 个已发布的植物数据集，我们能够使用 GetOrganelle 从 47 个数据集重新组装圆形质体。通过作图评估，GetOrganelle 组装比已发表的和/或 NOVOPlasty 重新组装的质体更准确。我们还使用 GetOrganelle 组装完整的线粒体基因组。 GetOrganelle 根据 GPL-3 许可证免费发布 (https://github.com/Kinggerm/GetOrganelle)。