Background: Nanotechnology is gaining emerging interest in advanced drug discovery therapeutics due to their tremendous properties including enhanced delivery of therapeutic payload, extensive surface to volume ratio, high permeability, retention behaviors, etc. The gold nanoparticles (AuNPs) are favored due to their advanced features, such as biogenic, tunable physiochemical response, ease in synthesis, and wide range of biomedical applications. The phytochemicals have been focused to design Au nano-carrier-based conjugation for active-targeting drug delivery due to their nano conjugation ability.
Aim: The present study describes the facile synthesis of 20nm spherical AuNPs and their conjugation with reported anti-cancer phytocompound Withanolide-A (1).
Methods: The 20nm sAuNPs were synthesized chemically and characterized their phytochemical gold nanoconjugates through UV-visible spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM) imaging techniques. The anti-cancer therapeutic potentials were tested with both nanoconjugates and pure WithanolideA (1) by using SKBR3 breast cancer cells line.
Results: The synthesized sAuNPs showed significant conjugation with Withanolide-A and showed stability. Furthermore, these Au nanoconjugates with Withanolide-A (1) significantly induce blockage of SKBR3 cell growth at half maximal active concentration that compared to pure Withanolide-A (1).
Conclusion: Our findings provide a foundation to further progress how they can overcome cancer drug resistance by conjugating active drugs in combination with AuNPs through optimizing the effective drug concentration and removing the surface barrier.



中文翻译：

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Withanolide-A 与 20nm sAuNPs 缀合物对 SKBR3 乳腺癌细胞系的合成、表征和抗癌治疗潜力

背景：纳米技术因其巨大的特性（包括增强的治疗有效载荷的传递、广泛的表面积与体积比、高渗透性、保留行为等）在先进的药物发现疗法中获得了新的兴趣。金纳米粒子 (AuNP) 由于其先进的特性而受到青睐具有生物源性、可调节的理化反应、易于合成和广泛的生物医学应用等特点。由于其纳米共轭能力，植物化学物质一直专注于设计基于 Au 纳米载体的共轭，用于主动靶向药物递送。
目的：本研究描述了 20nm 球形 AuNP 的简便合成及其与已报道的抗癌植物化合物 Withanolide-A (1) 的结合。
方法：20nm sAuNPs 是化学合成的，并通过紫外-可见光谱、动态光散射 (DLS) 和透射电子显微镜 (TEM) 成像技术对其植物化学金纳米缀合物进行了表征。使用 SKBR3 乳腺癌细胞系测试了纳米缀合物和纯 WithanolideA (1) 的抗癌治疗潜力。
结果：合成的 sAuNPs 与 Withanolide-A 有显着的共轭，并表现出稳定性。此外，与纯 Withanolide-A (1) 相比，这些带有 Withanolide-A (1) 的 Au 纳米缀合物在最大活性浓度的一半下显着诱导 SKBR3 细胞生长受阻。
结论：我们的研究结果为进一步研究如何通过优化有效药物浓度和去除表面屏障将活性药物与 AuNPs 结合来克服癌症耐药性提供了基础。