In this study, a method for the qualification and quantification of 4 psychoactive substances in tea using ultra high performance liquid chromatography coupled with the quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) has been developed. Tea samples were extracted by a 50% (v/v) methanol–water solution, and then separated by an ACQUITY UPLC BEH Shield RP18 column using a binary solvent system by gradient elution. The analytes were determined by Q-TOF/MS in TOFMS and information-dependent acquisition (IDA)-MS/MS mode. The results showed that the mass accuracy error of the 4 psychoactive substances were lower than 5.0 × 10⁻⁶, and a good linear relationship was observed in the range of 0.5–500 μg L⁻¹ and correlation coefficient was higher than 0.9990. The LOD was in the range of 0.005–0.020 mg kg⁻¹ and the LOQ was in the range of 0.010–0.040 mg kg⁻¹. The recovery of the method was in range of 80.14–93.25% with spike levels of 0.010–0.400 mg kg⁻¹, and relative standard deviations were lower than 10%. The method was simple, specific and reliable. It has been successfully used for the detection of 4 psychoactive substances in tea samples.

中文翻译：

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超高效液相色谱-四极杆飞行时间质谱法测定茶叶中的四种精神活性物质

在这项研究中，开发了一种使用超高效液相色谱结合四极杆飞行时间质谱（UPLC-Q-TOF / MS）对茶叶中4种精神活性物质进行鉴定和定量的方法。茶样品用50％（v / v）的甲醇水溶液萃取，然后使用二元溶剂系统通过ACQUITY UPLC BEH Shield RP18色谱柱进行分离，进行梯度洗脱。通过TOFMS中的Q-TOF / MS和信息相关采集（IDA）-MS / MS模式确定分析物。结果表明，四种精神活性物质的质量准确度误差均低于5.0×10 ^-6，在0.5–500μgL ^-1范围内观察到良好的线性关系。相关系数高于0.9990。LOD在0.005–0.020 mg kg ^-1的范围内，LOQ在0.010–0.040 mg kg ^-1的范围内。该方法的回收率在80.14–93.25％之间，峰值水平为0.010–0.400 mg kg ^-1，并且相对标准偏差低于10％。该方法简单，专一，可靠。它已成功用于茶叶样品中4种精神活性物质的检测。