Graphene oxide (GO) has recently been considered one of the most promising carbon derivatives in nanotechnology. It has many excellent features such as tumor targeting ability, biocompatibility and low toxicity. Therefore, we conjugated docetaxel (DTX) to GO-PEG molecule and investigate its anticancer efficacy in prostate cancer cell line (DU-145). In order to obtain GO-PEG-DTX molecules, we conjugated the DTX via bonds to PEG chains pegylated to the GO surface. We also investigated the stability of GO-PEG-DTX in different biological fluids such as cell mediums, PBS and water in vitro conditions. GO-PEG-DTX has the highest zeta potential in water. In the current research SEM, UV-Vis, and FTIR analyses and zeta potential were utilized for the characterization of nano-sized GO-PEG-DTX. Anticancer efficacy of GO-PEG-DTX were then investigated in DU-145 prostate cancer cell line using MTT metod. The prostate cancer cells were treated by different concentrations of GO-PEG-DTX, GO-PEG, GO, and DTX (1–100 µg/ml) during 24, 48 and 72 h. The spectrophotometric analyzed values at 570 nm were recorded and analysed with Graphpad Prism7. IC₅₀ growth inhibition values was determined. The data showed that the GO-PEG-DTX had a highly effective anticancer activity on prostate cancer cell lines after 24, 48 and 72 hours compared to other molecules. GO-PEG-DTX was found statistically significant in the DU-145 cell line (***p < 0.0001, **p > 0.001, and *p > 0.01). As a result, it can be said that PEGylated GO is an excellent nanocarrier system for the high anticancer activity of DTX. Loading of anticancer drugs using this type of graphene-based nano carrier and delivery to targeted tissues may find potential application in biomedicine.

氧化石墨烯（GO）最近被认为是纳米技术中最有前途的碳衍生物之一。它具有肿瘤靶向能力强、生物相容性好、毒性低等诸多优良特性。因此，我们将多西紫杉醇 (DTX) 与 GO-PEG 分子结合，并研究其在前列腺癌细胞系 (DU-145) 中的抗癌功效。为了获得 GO-PEG-DTX 分子，我们通过与聚乙二醇化到 GO 表面的 PEG 链的键将 DTX 共轭。我们还研究了 GO-PEG-DTX 在不同生物流体（如细胞培养基、PBS 和水）体外条件下的稳定性。GO-PEG-DTX 在水中具有最高的 zeta 电位。在目前的研究中，SEM、UV-Vis 和 FTIR 分析以及 zeta 电位被用于表征纳米尺寸的 GO-PEG-DTX。然后使用 MTT 方法在 DU-145 前列腺癌细胞系中研究 GO-PEG-DTX 的抗癌功效。在 24、48 和 72 小时内用不同浓度的 GO-PEG-DTX、GO-PEG、GO 和 DTX（1-100 µg/ml）处理前列腺癌细胞。使用 Graphpad Prism7 记录和分析 570 nm 处的分光光度分析值。我知道了测定了_{50 个}生长抑制值。数据显示，与其他分子相比，GO-PEG-DTX 在 24、48 和 72 小时后对前列腺癌细胞系具有高效的抗癌活性。发现 GO-PEG-DTX 在 DU-145 细胞系中具有统计学意义（***p < 0.0001、**p > 0.001 和 *p > 0.01）。因此，可以说聚乙二醇化的 GO 是一种优异的纳米载体系统，具有 DTX 的高抗癌活性。使用这种基于石墨烯的纳米载体装载抗癌药物并输送到目标组织可能会在生物医学中找到潜在的应用。